Abstract Number: PB0133
Meeting: ISTH 2020 Congress
Theme: Coagulation and Natural Anticoagulants » Animal Models in Thrombosis and Hemostasis
Background: Conditions with dehydration are associated with an increased risk for thrombosis. It has been shown that water-restriction in mice increases plasma von Willebrand factor (vWF) levels. Furthermore, there is evidence that dehydration increases the secretion of vWF by endothelial cells in-vitro.
Aims: As vWF is critical for the development of venous thrombosis in murine models, we tested the hypothesis that a high-salt diet, which induces renal water loss, also leads to a pro-thrombotic state in a murine model of venous thrombosis.
Methods: Starting at the age of 6 weeks, C57BL/6J mice were fed a high-salt diet (4% NaCl chow and 0,9% NaCl water). After four weeks, we performed subtotal vena cava inferior ligation and studied development of deep vein thrombosis via ultrasound and histology. We performed a short-time experiment over 3 days and a longer study over 21 days to depict thrombus formation and resolution. Blood counts and coagulation parameters were investigated after the experiments.
Results: After 3 days, we did not see differences in thrombus formation in mice on a high-salt diet. However, histological studies revealed a significant decrease of the fibrotic area of the thrombi in high salt fed mice. The long-term experiment confirmed these findings and showed that thrombus resolution was not altered by the high-salt diet as well. After 21 days of subtotal IVC-ligation, mice on high-salt showed a significant increase in platelet counts and fibrinogen levels. Furthermore, circulating neutrophils were increased, whereas erythrocyte counts were not affected.
Conclusions: Our findings indicate that a high-salt diet has effects on blood cells and fibrinogen, which could contribute to a pro-thrombotic state. However, these changes were not paralleled by increased thrombus formation in our model of subtotal vena cava inferior ligation. Further studies are required to clarify the influence of high salt diet on venous thrombosis.
To cite this abstract in AMA style:
Wild J, Knopp T, Molitor M, Hobohm L, Münzel T, Wenzel P, Karbach S. High Salt Intake Increases Platelet Counts and Plasma Fibrinogen Levels but Has No Effect on Thrombus Formation or Resolution in a Murine Model of Venous Thrombosis [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/high-salt-intake-increases-platelet-counts-and-plasma-fibrinogen-levels-but-has-no-effect-on-thrombus-formation-or-resolution-in-a-murine-model-of-venous-thrombosis/. Accessed May 6, 2021.« Back to ISTH 2020 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/high-salt-intake-increases-platelet-counts-and-plasma-fibrinogen-levels-but-has-no-effect-on-thrombus-formation-or-resolution-in-a-murine-model-of-venous-thrombosis/