Abstract Number: PB0464
Meeting: ISTH 2020 Congress
Theme: Diagnostics and OMICs » Biomarkers of Thrombosis and Hemostasis
Background: Cell membrane plays an essential role in the different reactions that occur during coagulation, highlighting the presence of phospholipids, lipids and glycolipids, with pro- and anticoagulant actions.
Aims: To identify, through an advanced lipidomics analysis, the main lipid groups associated with venous thromboembolic disease (VTE).
Methods: Plasma samples of 30 VTE patients and paired 30 healthy volunteers were selected. We analysed the lipids present in the plasma samples by LC-MS/MS. The annotation of the lipid groups was carried out using the specific package of R: Lipid MS. Results were analysed by logistic regression analysis. We also analysed the correlation between lipid groups and coagulation parameters.
Results: A total of 379 lipids were identified in our plasma samples. These lipids were grouped according to the lipid class to which they belonged, identifying a total of 18 classes of circulating lipids, of which 11 had a statistical difference between both groups. We obtained decreased levels of CEs and increased levels of carnitines, ceramides, DGs, FAs, LPGs, MGs, PCs, PEs, PGs and SMs in patients compared to controls (P < 0.05). In addition, we found that FAs and SMs were associated with thrombotic recurrence (P < 0.05). Next, we performed a predictive model with the lipid classes with the highest discriminatory capacity between both study groups (LPGs, FAs, SMs and ceramides), and obtained an AUC of 0.940 (95% CI: 0.87-1). Finally, we observed an association between some of the lipid classes and free circulating DNA in plasma.
Conclusions: We have identified 11 plasma lipid classes associated with VTE. These results suggest the existence of new molecules and routes of action involved in the coagulation system. It would be necessary to carry out further studies to identify the specific lipids responsible for the effect on thrombotic risk.
ISCIII(PI14/00512,FI14/00269,CPII15/00002,PI17/00495),FEDER,Generalitat Valenciana (ACIF/2017/138),IIS La Fe (2014/0718,2016/0820).
To cite this abstract in AMA style:
Fernandez-Pardo A, Oto J, Alcoriza M, Martos L, Plana E, Solmoirago MJ, Yelo M, Cana F, Ferrando F, Blanquer A, Bonanad S, España F, Medina P, Roca M, Navarro S. Identification of a Circulating Lipid Profile Associated with Venous Thromboembolism [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/identification-of-a-circulating-lipid-profile-associated-with-venous-thromboembolism/. Accessed April 17, 2024.« Back to ISTH 2020 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/identification-of-a-circulating-lipid-profile-associated-with-venous-thromboembolism/