ISTH Congress Abstracts

Official abstracts site for the ISTH Congress

MENU 
  • Home
  • Congress Archive
    • ISTH 2022 Congress
    • ISTH 2021 Congress
    • ISTH 2020 Congress
  • Resources
  • Search

Immunofluorescence Staining of Blood Smears for the Diagnostics of Platelet Disorders: A Single-center Experience in a Pediatric Hospital

N. Podoplelova1,2, E. Popova3,1, P. Zharkov2, D. Fedorova2, A. Greinacher4, M. Panteleev1,2,3

1Center for Theoretical Problems of Physicochemical Pharmacology, Moscow, Russian Federation, 2Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russian Federation, 3Lomonosov Moscow State University, Moscow, Russian Federation, 4Institut für Immunologie und Transfusionsmedizin, Universitätsmedizin Greifswald, Greifswald, Germany

Abstract Number: PB0872

Meeting: ISTH 2021 Congress

Theme: Platelet Disorders, von Willebrand Disease and Thrombotic Microangiopathies » Inherited Thrombocytopenias

Background: The method of immunofluorescence staining of blood smears is a recently developed (Greinacher et al. J Thromb Haemost 2017; 15: 1511-1521) approach of remote diagnostics of various platelet pathologies including MYH9 disorders/MYH9-related disease, biallelic Bernard-Soulier syndrome, Glanzmann thrombastenia, and gray platelet syndrome, and others. We report here experience of introducing this method in the National Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitry Rogachev (Moscow, Russia), which is the main national pediatric hematology hospital that provides diagnosis and therapy to children with blood disorders throughout the country.

Aims: Our study aimed to transfer this relatively labor-intensive and skill-sensitive methodology, to introduce in the routine laboratory practice, and to perform its validation.

Methods: Blood smears from healthy donors and patients were prepared using the standard method established worldwide, air-dried, and storage. Citrate anticoagulated blood was used for preparation. For immunofluorescence labeling was used primary antibodies (Myosin, LAMP 1 (H5G11), LAMP 2 (H4B4), VWF, P-Selectin (CD62P), CD63 (delta-granules), Ib/IX CD42a (FMC25), IIb/IIIa CD41 P2, β1-Tubulin (2.1.), α-Tubulin (RM113)) and fluorescence labeling secondary antibodies. Blood smears were assessed by immunofluorescence microscopy.

Results: Patients with different platelet disorders (9 patients with MYH9 disorder, 7 patients with Wiskott-Aldrich syndrome, 2 patients with Bernard-Soulier syndrome, 2 patients Hermansky-Pudlak syndrome,1 patient with gray platelets syndrome, 1 patient with Glanzmann thrombastenia) confirmed by the genetic analysis and clinical symptoms were assessed. In addition to the immunofluorescence assay, each patient was analyzed platelet function by flow cytometry and aggregation by standard methods. For all patients, we were able to identify the abnormalities by immunofluorescence associated with established diagnosis.
The study was supported by the grant of the President of the Russian Federation.

Conclusions: In this work it was determined that the immunofluorescence staining of blood smears enables us to receive reproducible results and implement the diagnostic of platelet disorders.

To cite this abstract in AMA style:

Podoplelova N, Popova E, Zharkov P, Fedorova D, Greinacher A, Panteleev M. Immunofluorescence Staining of Blood Smears for the Diagnostics of Platelet Disorders: A Single-center Experience in a Pediatric Hospital [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 2). https://abstracts.isth.org/abstract/immunofluorescence-staining-of-blood-smears-for-the-diagnostics-of-platelet-disorders-a-single-center-experience-in-a-pediatric-hospital/. Accessed September 27, 2023.

« Back to ISTH 2021 Congress

ISTH Congress Abstracts - https://abstracts.isth.org/abstract/immunofluorescence-staining-of-blood-smears-for-the-diagnostics-of-platelet-disorders-a-single-center-experience-in-a-pediatric-hospital/

Simple Search

Supported By:

Takeda logo

ISTH 2022 Congress site

Visit the official web site for the ISTH 2022 Virtual Congress »

  • Help & Support
  • About Us
  • Cookies & Privacy
  • Wiley Job Network
  • Terms & Conditions
  • Advertisers & Agents
Copyright © 2023 John Wiley & Sons, Inc. All Rights Reserved.
Wiley