Abstract Number: PB2562
Meeting: ISTH 2020 Congress
Background: Recurrent pregnancy loss (RPL) is a complex, multifactorial disease, with a frequency of 0.5-3 % in all couples trying to conceive. Low TFPI level increased risk of RPL in the West. However, the association of TFPI levels with its polymorphisms and their role in Indian RPL patients have not been studied yet.
Aims: To analyse the distribution of TFPI polymorphisms, their effects on TFPI levels and risk of RPL in India.
Methods: RPL women with at least 3 consecutive pregnancy losses before 20 week´s gestational age and equal number of healthy women with, at least one naturally conceived pregnancy studied. Plasma TFPI levels were determined by ELISA and its normal range determined (Mean±2SD of TFPI levels in controls). TFPI polymorphisms 287T/C, 536C/T and 399C>T were detected by PCR-RFLP and allele specific PCR respectively.
Results: 70 RPL patients, median age 34 years range (21-44 years) were recruited. Mean TFPI level was significantly lower in patients (37.21±13.86 ng/ml) than controls (48.04±13.30 ng/ml, p=0.001). Moreover, 8 patients with RPL had low TFPI (< 21.44 ng/ml), whereas no control had low TFPI. The prevalence of CT and TT genotypes of 399C>T polymorphism was significantly higher 22 (31.4%) in patients than 9 (12.8%) controls (p=0.01). 536C/T polymorphism was not detected in our studied population. The prevalence of 287T/C polymorphism was higher 19 (27.1%) in patients than 15 (21.4%) controls but difference was not statistically significant (p=0.55). Relation between the TFPI levels and their genotypes are shown in table.
Conclusions: Low TFPI level predisposes to RPL. 399C/T polymorphisms associated with low levels of TFPI and RPL. 287T/C polymorphism neither associated with TFPI level nor with risk of RPL. Thus, TFPI 399C/T polymorphisms detection may help in identifying high risk of RPL in India. A study with large sample size is required to confirm these findings.
|Polymorphisms||TFPI levels ng/ml (Mean±SD)||OR (95% CI)||ORA (95% CI)|
|399C/T CC CT TT||43.32±14.227 32.8 ±13.559 31.42±9.880 0.000*,0.001$,0.087#||1.0 3.384(0.63-17.99) 4.061(1.85- 8.89)||1.0 3.48(0.61-19.87) 4.72(2.02-11.01)|
|287T/C TT TC CC||43.77±13.15 38.89 ±14.71 37.51±13.83 0.100*, 0.109$, 0.478#||1.0 2.092(1.005-4.354) 3.254 (0.8312.74)||1.0 2.44(1.092-5.44) 3.85(0.92-16.11)|
[TFPI levels according to genotypes of TFPI polymorphisms and risk of RPL]
To cite this abstract in AMA style:Kishor K, Sharma A, Kumar R, Maharana S, Ranjan R, Mahapatra M, Tyagi S, Saxena R. Impact of 399C/T Polymorphism of Tissue Factor Pathway Inhibitor on Risk of Recurrent Pregnancy Loss in India [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/impact-of-399c-t-polymorphism-of-tissue-factor-pathway-inhibitor-on-risk-of-recurrent-pregnancy-loss-in-india/. Accessed October 19, 2021.
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