Abstract Number: PB1687
Meeting: ISTH 2020 Congress
Background: Previous studies show association between global platelet function, measured by the PFA-100 test, and risk of venous thromboembolic disease (VTE). This relation does not involve increased platelet aggregation, but changes in platelet adhesiveness have not been explored.
Aims: Evaluate the platelet adhesive function in patients with VTE.
Methods: Differences in platelet adhesion characteristics between VTE patients (n=54) and matched healthy individuals (n=51) were evaluated by using whole blood samples in: i) PFA-100, to measure global platelet function as closure times with epinephrine and ADP as inductors, ii) the IMPACT test, to evaluate platelet adhesiveness on a plastic surface (750 rpm), iii) microfluidic devices, to explore platelet adhesion on collagen type I (800 s-1) with 2D and 3D evaluation by confocal microscopy, and iv) membrane glycoprotein evaluation by flow cytometry. Plasma levels of VWF were measured.
Results: PFA-100 closure times were significantly shorter (p< 0.01) in patients than in controls for both, ADP and Epinephrine (70.5±11.2s vs 93.5±25.2s, and 78.4±13.5s vs 102.3±24.7s, respectively). The IMPACT test showed a significant increase in platelet aggregates size when using blood from VTE patients vs. controls (118.9±5.4 µm2 vs 100.2±5.7 µm2, p< 0.05, respectively) with a reduction in the interactions number (760.2±44.9 vs. 846.9±46). Platelet adhesion on collagen was enhanced area for VTE patients (16.8±0.9% vs. 13.3±0.8%, p< 0.05), with an increased aggregate volume (80.9±14.9µm3 vs 69.7±45.9, p< 0.05, n=5). Platelet glycoprotein expression was not altered in VTE patients. Plasma VWF levels were significant higher in VTE patients than in controls (238.3±13.8 vs 153.9±13.6, p< 0.01).
Conclusions: Platelets from VTE patients exhibit a proadhesive phenotype under flow conditions that could be related to the shortened occlusion times observed at the PFA-100. This enhanced adhesiveness may be explained by increased levels of VWF. Therefore, primary hemostasis plays a consistent role in venous patophysiology of VTE.
To cite this abstract in AMA style:Jerez D, Diaz-Ricart M, Arús M, Torramadé-Moix S, Molina P, Ramos A, Carrasco M, Villalta N, Martinez V, Llobet D, Sanchez P, Palomo M, Vidal F, Escolar G, Souto JC. Implication of Platelet Adhesiveness in the Risk of Venous Thromboembolic Disease [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/implication-of-platelet-adhesiveness-in-the-risk-of-venous-thromboembolic-disease/. Accessed September 29, 2023.
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