Abstract Number: PB1105
Meeting: ISTH 2022 Congress
Background: Assessment of P2Y12 therapeutic responsiveness is vital to preventing thrombotic and hemorrhagic complications in patients with cardiovascular diseases. Although various P2Y12 tests are being used at POC, the concentration of agonist used for each test varies greatly depending on the test equipment, resulting in even the opposite diagnosis result. We introduced a microfluidic cartridge Anysis-p2Y12 to assess P2Y12 inhibitor with minimum concentrations of ADP and PGE1.
Aims: This presentation describes the new Anysis-P2Y12 assay performance comparing with VerifyNow-P2Y12 in cardiac patients and analyzes the P2Y12 low-response rates of the two devices.
Methods: In total, 125 samples were collected from cardiac patients referred for a P2Y12 antiplatelet response test. The test result of Anysis-p2Y12 was the migration distance (MD) in the microfluidic system until the blood flow stops.
Results: The MDs without and with P2Y12 were 182±30 and 264±12mm, respectively (p < 0.0001). Compared to VerifyNow-P2Y12, the positive percent agreement rate and negative percent agreement of Anysis-200 were 96.8% and 88.7%, respectively. Cohen’s kappa coefficient between the two devices was 0.761. However, there was an apparent difference in the low-response rate to P2Y12, which was 36.5% for VerifyNow and 5.9% for Anysis. The different results in the low-response rate to P2Y12 was mainly due to the the concentration of agonist used for each test. In fact, the concentrations of ADP in VerifyNow were 20 uM, which is the 10-folds of that of Anysis-P2Y12 (2 uM).
Conclusion(s): The microfluidic platelet function assay, Anysis-P2Y12 adopted the recommended concentration of agonist and can help screen patients with abnormal P2Y12 non-responsiveness consistent with clinical results .
To cite this abstract in AMA style:Shin S, Kim S, Choi C, Piao J. In-vivo mimicking platelet thrombus formation in a microfluidic system (Anysis): Assessment of P2Y12 inhibitors for cardiology patients [abstract]. https://abstracts.isth.org/abstract/in-vivo-mimicking-platelet-thrombus-formation-in-a-microfluidic-system-anysis-assessment-of-p2y12-inhibitors-for-cardiology-patients/. Accessed March 4, 2024.
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