Abstract Number: PB0460
Meeting: ISTH 2021 Congress
Background: Activation of coagulation cascade involves activation of factor (F) XI and activated FXI (FXIa) was found in plasma of patients with thrombotic conditions and other conditions. Global hemostasis parameters are usually investigated in response to tissue factor (TF) trigger and there is limited-investigation of additional effect that can be caused by presence of FXIa.
Aims: To evaluate the influence of FXIa on fluorogenic thrombin generation (TG), clot formation and lysis under conditions of reduced FV, FVII, FVIII or FIX.
Methods: A dual channel fluorescence and turbidity microplate reader was used to simultaneously observe TG and clot formation and lysis, respectively. FV-, FVII-, FVIII-, and FIX-deficient plasmas were supplemented with their respective factors in the range of concentrations from 0.01 to 1 IU/ml. Coagulation was triggered with TF with or without FXIa in the presence of tissue plasminogen activator. Thrombin peak height (TPH) and the times to clot formation (ClotT), thrombin peak (PeakT) and clot lysis (LysisT) were obtained.
Results: In FVII- and FIX-deficient plasmas, FXIa addition increased the responses of TPH to FVIII and FIX in the concentrations of respective factors higher than 0.01 IU/ml. In FV- and FVII-deficient plasmas, FXIa changed TG, clot formation and lysis parameters at the concentrations of FV and FVII below 0.01 IU/ml. In FVII-deficient plasma, FXIa blocked the responses of ClotT, PeakT and LysisT to FVII and the correlation between ClotT and LysisT. The correlations between TPH and LysisT in FVIII- and FIX-deficient plasmas were weakened by FXIa. In FV-deficient plasma, there was no correlation between TG, clot formation and lysis parameters with or without FXIa.
Conclusions: Addition of FXIa to TF trigger reveals new relationships between TG and lysis parameters, offering improved differentiation of coagulopathies associated with coagulation factor deficiencies.
To cite this abstract in AMA style:Tarandovskiy I, Chang WC, Ovanesov M. Influence of Activated Factor XI on Simultaneous Thrombin Generation and Fibrin Clot Lysis in Factor Deficiencies [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 1). https://abstracts.isth.org/abstract/influence-of-activated-factor-xi-on-simultaneous-thrombin-generation-and-fibrin-clot-lysis-in-factor-deficiencies/. Accessed September 16, 2021.
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