Abstract Number: LPB0131
Meeting: ISTH 2021 Congress
Background: The activation of platelets is caused by their immediate contact with any non-physiological surfaces. Understanding the mechanism and repercussions of such interaction is crucial in the context of biomedical research. Selection of a platelet congenial surface is of utmost importance in manufacturing platelet storage bags or even in fabricating an anti-thrombogenic implant surface. Platelet-surface activation can be inhibited by modulating the surface properties.
Aims: We developed agarose and gelatin-based hydrogel films and their nanocomposites to inhibit platelet-surface adhesion.
Methods: The fabricated hydrogel films were characterized by contact angle measurements using the captive bubble technique while the swelling behavior and water retention properties of the hydrogels and their nanocomposite films were investigated to determine the stability of the films. The stiffness of the films was measured by using AFM based indentation technique. The de-adhesion forces between platelets and surfaces were measured in real-time by using platelet force spectroscopy using FluidFM. Platelet morphologies on the gels were obtained by confocal laser microscopy and scanning electron microscopy.
Results: Agarose films exhibit higher surface wettability, better controlled-swelling properties, and greater stiffness compared to gelatin, resulting in a strong reduction of platelet adhesion. The mechanical property and surface wettability of the hydrogel films was varied by adding magnetite (Fe3O4) nanoparticles, however, degree of platelet adhesion did not change.
Conclusions: Agarose and agarose nanocomposite materials strongly reduce platelet adhesion and spread. As several types of nanoparticles carry anti-bacterial properties, agarose nanocomposite can be a promising candidate in the fabrication of platelet storage bags that contains anti-bacterial functions.
To cite this abstract in AMA style:Apte G, Lindenbauer A, Schemberg J, Rothe H, Nguyen T-. Inhibition of Surface-induced Platelet Activation on Hydrogel Films [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 1). https://abstracts.isth.org/abstract/inhibition-of-surface-induced-platelet-activation-on-hydrogel-films/. Accessed September 24, 2021.
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