Abstract Number: PB1006
Meeting: ISTH 2021 Congress
Background: Biomaterial-blood interactions are critical for the successful tissue integration of implants and might be used to guide wound healing. Fibrin(ogen) mediates platelet aggregation and thrombus formation at vascular injury sites. Studies have shown that the local adhesion protein density and substrate stiffness inform platelet responses, yet the influence of fibrin(ogen) topography is poorly understood.
Aims: To investigate platelet adhesion, secretion, and procoagulant activity upon interaction with fibrinogen scaffolds of different nanotopographies.
Methods: Platelet-rich plasma (PRP) or washed platelets were incubated on planar or nanofibrous fibrinogen scaffolds prepared by salt-induced self-assembly (Stapelfeldt et al., Biofabrication 2019; Stapelfeldt et al., Nanolett 2019), as well as on control fibrinogen-coated coverslips. Samples were fixed and stained for confocal microscopy to evaluate platelet activation in terms of cytoskeleton adhesion morphology (F-actin organization), α-granule secretion (P-selectin surface expression), and procoagulant activity (phosphatidylserine exposure).
Results: Platelets in PRP remained round on adsorbed or planar fibrinogen but spread and formed small clots on nanofiber scaffolds. In contrast, washed platelets adhered and spread on all substrates and developed similar adhesion morphologies. Most platelets on all substrates were P-selectin positive but showed heterogeneous variation in phosphatidylserine exposure, independent of the presence of plasma proteins.
Conclusions: These preliminary results suggest that platelets interact more strongly with 3D fibrinogen scaffolds than 2D fibrinogen coatings. Further investigation is required to elucidate the molecular mechanisms underpinning platelet behaviour on 3D substrates.
To cite this abstract in AMA style:Kenny M, Stamboroski S, Taher R, Brüggemann D, Schoen I. Interaction of Blood Platelets with Engineered Fibrinogen Scaffolds of Different Topographies [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 1). https://abstracts.isth.org/abstract/interaction-of-blood-platelets-with-engineered-fibrinogen-scaffolds-of-different-topographies/. Accessed September 24, 2021.
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