Abstract Number: PB0695
Meeting: ISTH 2020 Congress
Background: The C – reactive protein (CRP) is an acute-phase protein with an affinity to phospholipids. Some studies correlate a prolongation of the Activated Partial Thromboplastin Time (APTT) in proportion to the increase in the CRP concentration depending on the type of APTT reagent used in the routine.
Aims: To evaluate the interference of the levels of CRP in the reagent used for the determination of APTT performed routinely in our laboratory.
Methods: Samples from 1859 patients were included in this study. APTT assays were performed on the ACL TOP 750 instrument using the APTT-SP HemosiL (Werfen). Normal samples showed normal APTT ratio up to 1.19 and the abnormal APTT ratio were higher than 1.19. CRP measurements were performed on VITROS 5600® using the hs-CRP and CRP reagents (Ortho Clinical Diagnostics). The CRP results were classified according to the patient´s cardiovascular risk (Low: < to 1.0 mg / L; Medium: between 1.0 and 3.0 mg / L; High: > to 3.0 mg / L ). The Spearman test was used to assess the correlation between the levels of CRP and APTT ratio.
Results: In total samples evaluated, 18.61% had high risk CRP and prolonged APTT ratio; 57.27% had high risk CRP with normal APTT ratio, 1.24% medium risk CRP and prolonged APTT ratio; 10.99% CRP medium risk and normal APTT ratio; 0.73% CRP low risk and prolonged APTT ratio and 11.16% CRP low risk and normal APTT ratio. There was no correlation between the levels of CRP with abnormal APTT ratio (Spearman r = 0.27; r2 = 0.055)
Conclusions: The data obtained in this study show that high levels of CRP did not influence the prolongation of APTT assay in our routine, not showing in this study interference with the phospholipids present in the evaluated reagent.
To cite this abstract in AMA style:Farana Matos M, Finazzo C, Taniguchi E. Interference of C – Reactive Protein Level in Activated Partial Thromboplastin Time [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/interference-of-c-reactive-protein-level-in-activated-partial-thromboplastin-time/. Accessed October 19, 2021.
« Back to ISTH 2020 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/interference-of-c-reactive-protein-level-in-activated-partial-thromboplastin-time/