Abstract Number: PB0010
Meeting: ISTH 2021 Congress
Background: Erythrocytes gas transport function controls the diffusion of chloride and bicarbonate through erythrocyte membrane and limits the rate of CO2 transfer from tissues to lungs. Detailed characterization of morpho-functional erythrocyte parameters is necessary to control risk of hypoxic stage in organism. This is especially important in case of cardiovascular diseases. It can lead to formation of unstable atheroma in places with increased oxygen demand.
Aims: This study aims to verify the hypothesis that deviations in erythrocyte parameters (anion permeability) correlate with atheroma types (stable or unstable).
Methods: Carotid artery plaques were obtained by endarterectomy and measured by AxioCam MRc5. Whole blood was taken by venopuncture. Then cells were used in experiments at room temperature (22 °C). All donors were divided in three groups: patients with stable and unstable plaques and healthy donors. Experiments were carried out on scanning flow cytometer (fabricated by CytoNova Ltd., Novosibirsk, Russia, http://cyto.kinetics.nsc.ru) which permit to determine characteristics of individual blood cells.
Results: We obtained for each measured RBC in samples the Hb content simultaneously with morphological characteristics. We define the anion permeability in terms of mean number of ‘effective’ (parameter derived from erythrocyte isotonic lysis in ammonium chloride) and ‘activated’ (parameter derived from addition of nifedipine solution before isotonic hemolysis) erythrocyte Band 3 protein.
Total 45 patients and 45 donors were included in this study. It turns out that patients with stable atherosclerotic plaque have increased ratio between ‘activated’ and ‘effective’ anion exchangers compare to even healthy group. Patients with unstable plaque have significant nonactive fraction of anion carriers (up to 80%).
Conclusions: Deviations in gas transport function can be explained as the protection mechanism from hypoxic conditions in places with increased oxygen demand, such as atherosclerotic plaque. And, consequently, decreased anion exchange can be a predictor of atheroma destabilization.
To cite this abstract in AMA style:Yastrebova E, Karpenko A. Investigation of Red Blood Cells Influence on Atherosclerotic Plaque by Scanning Flow Cytometry [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 2). https://abstracts.isth.org/abstract/investigation-of-red-blood-cells-influence-on-atherosclerotic-plaque-by-scanning-flow-cytometry/. Accessed August 15, 2022.
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