Abstract Number: PO101
Meeting: ISTH 2021 Congress
Background: The management of advanced atherosclerotic stenosis reduces the risk of stroke and myocardial infarction and their related deaths. The laser atherectomy methods that are currently in use, cause to inflammation and subsequent restenosis.
Aims: The aim of this study was to evaluate the effect of Lipid- based encapsulated methotrexate nanoparticles – mediated ultrasound sonoporation therapy on inflammation and intimal hyperplasia reduction after laser angioplasty in the hamster abdominal aorta model of fibrocalcific atherosclerosis (fibrotic plaque with a large calcified content), wherein diagnostic B- mode ultrasound is combined with sonoporation therapy system, with a goal of increased safety.
Methods: Golden Syrian Hamsters were submitted to the fibrocalcific atherosclerosis at the abdominal aorta (approximately 0.5 cm superior to the iliac bifurcation). Histopathology results showed the formation of advanced atherosclerosis with fibrous- derived extracellular matrix- rich plaque and calcification, resulted to severe stenosis (> 80%) in all of the hamsters’ arteries. Then treatment group underwent ArF excimer laser (193 nm) angioplasty followed by low- level focused ultrasound (F= 1.2 MHz, I= 10 J/cm2, PD= 150 ms)- mediated sonoporation therapy in combination with lipid- based encapsulated methotrexate nanoparticles (10 mg/kg) administration.
Results: Results from histopathology showed a significant reduction in the mean value for macrophages and smooth muscle hyperplasia cells density after laser thermal angioplasty in the sonoporation therapy group compared with the other groups (p< 0.05).
Conclusions: Enhanced apoptotic and anti- proliferative effect of methotrexate – induced by enhanced sonoporation effect of focused ultrasound, due to inertial cavitation- mediated collapsed capsules, can cause to reduce inflammation and restenosis after ArF excimer laser angioplasty of fibrocalcific atherosclerosis.
To cite this abstract in AMA style:Mehrad H, Foletti A. Lipid-based Encapsulated Methotrexate Nanoparticles-mediated Low Level Focused Ultrasound Sonoporation Therapy Decrease Inflammation and Restenosis after ArF Excimer Laser Angioplasty of Fibrocalcific Atherosclerosis [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 2). https://abstracts.isth.org/abstract/lipid-based-encapsulated-methotrexate-nanoparticles-mediated-low-level-focused-ultrasound-sonoporation-therapy-decrease-inflammation-and-restenosis-after-arf-excimer-laser-angioplasty-of-fibrocalcific/. Accessed March 4, 2024.
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ISTH Congress Abstracts - https://abstracts.isth.org/abstract/lipid-based-encapsulated-methotrexate-nanoparticles-mediated-low-level-focused-ultrasound-sonoporation-therapy-decrease-inflammation-and-restenosis-after-arf-excimer-laser-angioplasty-of-fibrocalcific/