Abstract Number: PB0511
Meeting: ISTH 2020 Congress
Background: Light transmission aggregometry (LTA) is a highly informative method, but has some limitations. A recently developed assay evaluates platelet aggregation in a diluted sample using low-angle light scattering, which has low sample volume requirement, provides additional information about aggregate structure and platelet shape change depending on the scattering angle, and uses standardized buffer as a medium.
Aims: Validation of the low-angle light scattering method for measurement of platelet aggregation.
Methods: Platelets were diluted by modified HEPES buffer to 10,000 platelets/ µl, stimulated by 800 nM ADP, and the initial velocity and amplitude of aggregation at 1.5° and 12° were evaluated. The study included healthy adults (n = 32, 21-40 years old) and healthy children (n = 20, 1-18 years old), pediatric patients with Glanzmann’s thrombasthenia (TG, n = 2), hypo- and dysfibrinogenemia (n = 1), as well as patients with bleedings (n = 19).
Results: We obtained reference intervals for healthy adults and children (Table 1). There were no significant differences in the parameters characterizing platelet aggregation and shape change between these control groups. The aggregation amplitudes obtained by low-angle light scattering method and LTA moderately correlated both in the group of healthy children and patients (Spearman´s correlation coefficient 0.47 and 0.44 respectively, p < 0.05). For patients with TG, hypo- and dysfibrinogenemia, there was shape change but no aggregation in response to ADP stimulation. The intake of a fat meal or physical activity before blood sampling, as well as the blood: citrate ratio did not affect the results of the study.
Conclusions: Our results show that the low-angle light scattering method can be used to characterize platelet aggregation: the influence of preanalytical variables has been evaluated; reference intervals were determined for healthy adults and children; the method has been tested on patients with bleeding.
|Initial velocity at 1.5°, AU||Amplitude of aggregation at 1.5°, AU||Amplitude of aggregation at 1.5°, AU|
|Adults||0.94 – 1.98||414 – 1159||1.88 – 2.63|
|Children||1.05 – 2.06||378 – 1008||1.72 – 2.53|
[Reference intervals for healthy adults and healthy children]
To cite this abstract in AMA style:Svidelskaya G, Ignatova A, Poletaev A, Seregina E, Zharkov P, Mindukshev I, Gambaryan S, Panteleev M. Low-Angle Light Scattering for the Analysis of the Platelet Functions: Preanalytical Variables and Evaluation in Pediatric Patients [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/low-angle-light-scattering-for-the-analysis-of-the-platelet-functions-preanalytical-variables-and-evaluation-in-pediatric-patients/. Accessed November 29, 2023.
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