Abstract Number: PB0365
Meeting: ISTH 2020 Congress
Background: Circulating cell-derived microparticles (MP) are important players in thrombogenesis because they carry tissue factor (TF), the initiator of blood coagulation. They are formed from membrane blebs of blood cells that are released from the cell surface by proteolytic cleavage of the cytoskeleton. All MPs are procoagulant because they provide a negative charged membrane surface for the assembly of coagulation factors.
Aims: With this study we performed thrombin generation assays on hypercoagulable plasma samples received at our Special Haemostasis Laboratory of the National Health Laboratory Service of South Africa. We isolated microparticles from these plasma samples and did thrombin generation assays again on these plasma samples without microparticles. We then determined the contribution of microparticles to the thrombin generation of these hypercoagulable plasma samples.
Methods: Microparticles were isolated from 50 hypercoagulable plasma samples and 50 normal plasma samples (control group), using the Ceveron microparticle filtration unit (MFU 500, Technoclone, Austria). Thrombin generation assays (TGA) were performed using the Technoclone Ceveron Alpha instument. Thrombin generation were measured over 90 minutes in both normal plasma samples and hypercoagulable plasma samples (with and without the presence of MPs). We calculated the peak thrombin concentration, velocity index and aeria under the curve.
Results: MPs from all hypercoagulable plasma samples exhibited increased thrombin generation with all TGA parameters. Microparticles contributed 49 ± 16% to the peak thrombin, 63 ± 19% to the velocity-index and 24 ± 10% to the AUC. Interesting to note is that MPs did not contribute at all to thrombin generation in normal plasma samples.
Conclusions: Microparticle mediated TGA is a novel way to assess functional procoagulant activity of MPs. Enhanced MP-mediated TGA was demonstrated in hypercoagulable plasma samples. These findings support a major role of MP in thrombogenesis.
To cite this abstract in AMA style:Meiring M, Ohaegbulam M. Microparticles Contribute to Hypercoagulation, but Not to Coagulation in Healthy Controls [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/microparticles-contribute-to-hypercoagulation-but-not-to-coagulation-in-healthy-controls/. Accessed August 15, 2022.
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