Abstract Number: PB1643
Meeting: ISTH 2020 Congress
Background: Quality management of blood platelet products is important for successful platelet transfusion therapy.
Although function of stored platelets is usually assessed by platelet aggregation ability, morphological change and P-selectin expression, senescence markers such as exposure of phosphatidylserine (PS) and microparticle (MP) have not been well evaluated in stored platelets.
In the previous study, we demonstrated that the number of PS-exposed platelets and MP were time-dependently increased during storage of platelets. Furthermore, activated partial thromboplastin time (APTT) was accelerated by the addition of PS-exposed platelets. However, the function of MP released from stored platelets is still unknown.
Aims: The aim of this study was to elucidate the role of MP released from stored platelets.
Methods: Expression of P-selectin, PS exposure and MP release were assayed by using flow cytometry after platelet rich plasma (PRP) were stored at 22℃ for 0-14 days with continuous gentle agitation.
Results: Initial pH in stored platelets was pH 7.51±0.38 and sustained from day0 to day14. The population of P-selectin-expressing platelets significantly increased in a time-dependent manner during incubation (day 0, 8.8%; day10, 37.8%). Similar results were obtained for PS-exposed platelets. MPs were not detected at day 0, but they appeared at day 3 and gradually increased thereafter. Interestingly, PS-exposure was detected on MPs like platelets after incubation, suggesting a functional role of PS-exposed MP in coagulation as observed for PS-exposed platelets in my previous study. Actually, coagulation time was significantly shortened by the addition of PS-exposed MP to the APTT assay.
Conclusions: PS-exposed MPs are released from stored platelets and accelerate coagulation activity.
To cite this abstract in AMA style:Noboruo I, Yamamoto T, Tanabe K, Kawaguchi T, Ikeda K, Kozuma Y. Microparticles Released from Stored Platelet Increase Coagulation Ability [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/microparticles-released-from-stored-platelet-increase-coagulation-ability/. Accessed November 29, 2023.
« Back to ISTH 2020 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/microparticles-released-from-stored-platelet-increase-coagulation-ability/