Abstract Number: PB0024
Meeting: ISTH 2021 Congress
Background: Ischemic stroke (IS) is one of the most frequent causes of death. In 2018, for every 6 deaths from cardiovascular disease, one was caused by stroke. Although there are well established treatment strategies diminishing post-stroke cell death, there are no available long-term diagnostic and prognostic methods to predict stroke severity. MicroRNAs (miRNAs) are a class of endogenous, non-coding RNA molecules. Since miRNAs have been illustrated to play an important role in various processes through regulation of multiple genes, and platelet function.
Aims: Analysis of plasma platelet-derived miR-19a-3p and miR-186-5p expression levels 24-h and 7-days after IS as novel diagnostic and prognostic biomarkers.
Methods: Blood samples of 28 patients diagnosed with acute IS with hyper platelet reactivity were collected 24-h and 7-days after stroke and 26 age- and gender-matched individuals free of stroke with multiple risk factors for cardiovascular disease. PlasmaRNA was extracted: mirVANAPARISKit; quality RNA was assessed: fluorometric assay; microarray: Affymetrix platform; RT-PCR for validation the miRNAs with Taqman advanced. p<0.05 (SPSS, IBM Corporation, Chicago, USA).
Results: MiR-186-5p levels were significantly lower in the study group 7 days post-stroke vs. in controls. MiR-19a-3p levels were significantly lower 7 days post-stroke vs. at 1 day of stroke and significantly higher among patients with moderate vs. minor stroke at 1 day of IS.
Conclusions: Our analysis showed alteration of circulating miRNAs after IS. MiR-19a-3p might have a predictive value for stroke severity prognosis and miR-186-5p might be a novel diagnostic biomarker for patients IS with hyper platelet reactivity.
To cite this abstract in AMA style:Eyileten C, Jakubik D, Jarosz-Popek J, Czajka P, Wolska M, Fitas A, Nowak A, Wicik Z, Mirowska-Guzel D, Członkowska A, Postuła M. miR-19a-3p Predicts Stroke Severity and miR-186-5p Might Be a Diagnostic Biomarker in Ischemic Stroke Patients with Hyper Platelet Reactivity [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 2). https://abstracts.isth.org/abstract/mir-19a-3p-predicts-stroke-severity-and-mir-186-5p-might-be-a-diagnostic-biomarker-in-ischemic-stroke-patients-with-hyper-platelet-reactivity/. Accessed October 20, 2021.
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