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miR-19a-3p Predicts Stroke Severity and miR-186-5p Might Be a Diagnostic Biomarker in Ischemic Stroke Patients with Hyper Platelet Reactivity

1Center for Preclinical Research and Technology CEPT, Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Warsaw, Poland, 2Doctoral School, Medical University of Warsaw, Warsaw, Poland, 3Centro de Matemática, Computação e Cognição, Universidade Federal do ABC, São Paulo, Brazil, 42nd Department of Neurology, Institute of Psychiatry and Neurology, Warsaw, Poland

Abstract Number: PB0024

Meeting: ISTH 2021 Congress

Theme: Arterial Thromboembolism » Cerebrovascular Disorders

Background: Ischemic stroke (IS) is one of the most frequent causes of death. In 2018, for every 6 deaths from cardiovascular disease, one was caused by stroke. Although there are well established treatment strategies diminishing post-stroke cell death, there are no available long-term diagnostic and prognostic methods to predict stroke severity. MicroRNAs (miRNAs) are a class of endogenous, non-coding RNA molecules. Since miRNAs have been illustrated to play an important role in various processes through regulation of multiple genes, and platelet function.

Aims: Analysis of plasma platelet-derived miR-19a-3p and miR-186-5p expression levels 24-h and 7-days after IS as novel diagnostic and prognostic biomarkers.

Methods: Blood samples of 28 patients diagnosed with acute IS with hyper platelet reactivity were collected 24-h and 7-days after stroke and 26 age- and gender-matched individuals free of stroke with multiple risk factors for cardiovascular disease. PlasmaRNA was extracted: mirVANAPARISKit; quality RNA was assessed: fluorometric assay; microarray: Affymetrix platform; RT-PCR for validation the miRNAs with Taqman advanced. p<0.05 (SPSS, IBM Corporation, Chicago, USA).

Results: MiR-186-5p levels were significantly lower in the study group 7 days post-stroke vs. in controls. MiR-19a-3p levels were significantly lower 7 days post-stroke  vs. at 1 day of stroke and significantly higher among patients with moderate vs. minor stroke at 1 day of IS.

MiRNAs expression difference between groups and miRNAs expression difference based on stroke severity. a) miR-186-5p; b) miR-19a-3p c) miR-19a-3p acute stroke; d) miR-19a-3p 7 days after stroke; e) miR-186-5p acute stroke; f) miR-186-5p 7 days after stroke. The initial stroke severity as measured by the NIHSS at admission. Minor stroke: 1-4 and moderate stroke: 5-15; p<0.05. NS: non significant
Receiver operating characteristic (ROC) curves of miR-19a-3p and miR-186-5p at the day-1 and day-7 stroke for prediction of stroke severity.

Conclusions: Our analysis showed alteration of circulating miRNAs after IS. MiR-19a-3p might have a predictive value for stroke severity prognosis and miR-186-5p might be a novel diagnostic biomarker for patients IS with hyper platelet reactivity.

To cite this abstract in AMA style:

Eyileten C, Jakubik D, Jarosz-Popek J, Czajka P, Wolska M, Fitas A, Nowak A, Wicik Z, Mirowska-Guzel D, Członkowska A, Postuła M. miR-19a-3p Predicts Stroke Severity and miR-186-5p Might Be a Diagnostic Biomarker in Ischemic Stroke Patients with Hyper Platelet Reactivity [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 2). https://abstracts.isth.org/abstract/mir-19a-3p-predicts-stroke-severity-and-mir-186-5p-might-be-a-diagnostic-biomarker-in-ischemic-stroke-patients-with-hyper-platelet-reactivity/. Accessed November 28, 2023.

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