Abstract Number: PB0739
Meeting: ISTH 2020 Congress
Background: We have reported on the properties of nattokinase (NK) produced by fermentation of Bacillus subtilis natto. In clinical practice, it has been found that it is effective as an antihypertensive and, more recently, has the potential to prevent dementia.
Aims: This time, it was revealed that NK has kinin production from kininogen and melanin aggregation inhibitory activity.
Methods: NK is a high-purity (423 IU/g) substance provided by (Honda Trading) Organo Food Teck Co. Ltd., Japan. The activity was measured by a well plate method using a synthetic substrate (Bz-Ile-Glu-(OR)-Gly-Arg-pNA:S-2222). The kinin-forming ability of NK was tested by the Magnus method using rat uterus as usual. B16 cells, a mouse melanoma cell line, were cultured in D-MEM supplemented with 10% fetal calf serum, 0.05% L-glutamine, and 0.2% sodium bicarbonate at 37oC and 5%CO2 for 25cm2. Propagation and subculture were performed using a culture flask. Total RNA was extracted using the AGPC (Acid-Guanidium-Phenol-Chloroform) method.
Results: When NK was applied to plasma kininogen, strong kinin activity was observed even at 1ng/ml or less. In addition, tyrosinase was added to a mixed solution of NK and DOPA (0.05%), and incubation revealed that the melanin aggregation reaction was inhibited. NK was found to efficiently inhibit tyrosinase synthesis using L-tyrosine as a substrate (Ki =1.17×10-6M). It also acted on mouse melanoma B16 producing cells, which are melanin-synthesizing cells, and the inhibition was confirmed by Western blotting or tyrosinase mRNA expression.
Conclusions: NK is a fibrinolytic enzyme, which has been found to have a direct kinin-producing effect. Kallikrein was thought to work to improve circulation. In addition, we have confirmed for the first time that NK has an effect of suppressing melanin synthesis.
To cite this abstract in AMA style:Sumi H, Yanagisawa Y, Naito S, Yatagai C. Nattokinase – Inhibition of Melanin Synthesis (Whitening Effect) [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/nattokinase-inhibition-of-melanin-synthesis-whitening-effect/. Accessed December 5, 2021.
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