Abstract Number: PB0170
Meeting: ISTH 2020 Congress
Background: The limitations of present established allopathic formulations for antithrombotic therapies has resulted in surge of natural or herbal based medications to find out potential treatment and cure for the ailment. The on-going studies reveal that traditional natural compounds possess remarkable antithrombotic properties both in clinical and experimental phases. However, their crude extract from plants may act on multiple targets exerting pleiotropic and synergistic effect making the mechanism of action more complex
Aims: The aim of present study is to explore the potential of natural compounds as oral anticoagulants and revealing the under lying molecular targets and mechanisms.
Methods: Pertaining above hypothesis, a natural compound library was prepared. The library consisted of 158 distinct compounds after screening 90,000 compounds. Each compound was docked with identified molecular target i.e. FXa, which is a FDA approved molecular target for thrombosis. The blind docking study gave 11 natural compounds as probable leads that could inhibit FXa. These leads were further analysed by SWISS-ADME and FXa activity assay. The four compounds, pravastatin, wogonin, hyperoside and diosmetin qualified above assays. They were further investigated by FES and CD for the binding. Wogonin and pravastatin based on FES and CD profile were tested in IVC ligated murine model.
Results: Pravastatin, an already reported drug for atherosclerosis is found to inhibit FXa and decrease platelet activation. It also showed decrease in thrombus size in IVC (inferior vena cava) ligated animals injected with pravastatin as compared to IVC controls.
Conclusions: In our study we tried to explore natural compounds as new anticoagulants or antithrombotic drugs. Our study based on in silico and in vivo animal work reveals for the first that pravastatin is a promising advancement as pharmacological agents available for thrombosis
To cite this abstract in AMA style:Sharma S, Garg I, Bhakuni T, Jairajpuri MA, Kumar B, Asharf MZ. Natural Compounds as Anticoagulant Drug: A New Avenue for Improving Thrombosis Outcome [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/natural-compounds-as-anticoagulant-drug-a-new-avenue-for-improving-thrombosis-outcome/. Accessed December 5, 2021.
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