Abstract Number: PB0392
Meeting: ISTH 2020 Congress
Background: Neutrophil extracellular traps (NETs) are extracellular neutrophil DNA fibers that bind chromatin proteins (Histones H3 and 4) and granule proteins (myeloperoxidase and elastase) formed during neutrophil activation. It was established in the literature that tumors activate neutrophils to induce NETosis promoting metastatic progression and activation of coagulation. Extensive NET formation was evidenced in peritoneal colon cancer metastases in mice and patients and DNase administration in animal models markedly reduced the number of metastases in animals. In addition, NET is responsible of chemokine-dependent colon cancer cell migration and adhesion in vitro.
Aims: we were interested in evaluating before surgery different constituents of NETs in plasma of patients with peritoneal cancer of various origins.
Methods: Adult patients with peritoneal carcinomatosis were studied just before surgery. They were classified according to the origin of peritoneal carcinomatosis. All patients provided written or oral consent for participation in NET levels in accordance with local ethics committee requirements. dsDNA was measured using Quant-iT Picogreen dsDNA assay kit, (Thermo Fisher Scientific), Histones (H3 and H4) were evaluated by ELISA Kits (Clinisciences) and myeloperoxidase (MPO) was evaluated by ELISA kit (Biotechne).
NET in plasma was identified by confocal microscopy after staining by Picogreen.
Results: The different parameters of NETs were evaluated in the plasma of 35 patients with peritoneal carcinomatosis of several origins (peritoneal or ovarian n=10, mesothelioma n=3; pseudomyxoma n=6, colon n=12, oesophagus n=1 and stomach n=1). In almost all cases, H4, H3 and DNA levels were increased whereas that of MPO was increased in only 67% of cases. DNA filaments were found in plasma from cancer patients.
Conclusions: Many constituents of NETs being increased in the plasma of these patients whatever the origin of peritoneal carcimatosis, it should be interesting to analyze the evolution of the disease in these patients.
To cite this abstract in AMA style:Mirshahi S, Aldybiat I, Contant G, Pocard M, Soria J, Mirshahi M. Neutrophil Extracellular Trap in Patients with Peritoneal Carcinomatosis of Several Origins [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/neutrophil-extracellular-trap-in-patients-with-peritoneal-carcinomatosis-of-several-origins/. Accessed February 25, 2024.
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