Abstract Number: PB2018
Meeting: ISTH 2020 Congress
Background: Several different host cells found in the tumor microenvironment, including immune cells such as neutrophils, seem to facilitate tumor progression. Neutrophils may undergo a cellular program that leads to the extrusion of a web-like material named Neutrophil Extracellular Traps (NETs) that are composed of DNA and proteins such as elastase, histones, and myeloperoxidase. NETs have been proposed as possible effectors in the tumor progression.
Aims: Herein, we evaluated the effects of isolated NETs on the human breast cancer cell line, MCF7.
Methods: Neutrophils were isolated from the blood of health donors and further stimulated with PMA to generate NETs. MCF7 cells were starved in serum-free medium, incubated with NETs for 16h, and samples were processed for qPCR, flow cytometry, and fluorescence microscopy analyses.
Results: Treatment of MCF7 with purified NETs promoted remarkable morphological changes in the tumor cells. In addition, the incubation of MCF7 with NETs dramatically increased the migratory properties of the tumor cells. NETs upregulated the mRNA expression of interleukin-6, interleukin-8 and matrix metalloprotease 9 (MMP9) genes, as assessed by real-time qPCR analyses, which was approximately 10, 12 and 100-fold higher than the control condition, respectively. In order to evaluate if NETs could promote β-catenin translocation from the membrane to the nucleus, we employed immunocytochemistry assays. NETs promoted the nuclear localization of β-catenin in MCF7 cells. Morevover, the expression of the cancer stem cell marker, CD44, was upregulated upon incubation of MCF7 cells with NETs, as demonstrated by qPCR and by flow cytometry analyses.
Conclusions: Collectively our data demonstrate that NETs increase the pro-tumoral properties of the human carcinoma cell line, MCF7. We propose that NETs may participate in the primary breast cancer progression.
To cite this abstract in AMA style:Cardoso K, Almeida V, Bagri K, Rossi M-, Mermelstein C, König S, Monteiro R. Neutrophil Extracellular Traps (Nets) Increase the Pro-Tumoral Properties of the Human Breast Cancer Cell Line, MCF7 [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/neutrophil-extracellular-traps-nets-increase-the-pro-tumoral-properties-of-the-human-breast-cancer-cell-line-mcf7/. Accessed February 20, 2024.
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