Abstract Number: PB0021
Meeting: ISTH 2020 Congress
Background: Clinically relevant animal models are required to better investigate pharmaceutical and surgical treatment of arterial thrombosis. Myocardial infarction (MI) caused by coronary arterial thrombosis, is frequent in patients with a rupture or injury of an atherosclerotic coronary arterial plaque. This occurs following a percutaneous coronary intervention or is the result of having acute coronary syndrome.
Aims: Our goal was to validate a model of acute MI induced by topical application of ferric chloride (FeCl3) solution on the left anterior descending (LAD) coronary of anesthetized rats.
Methods: Acute MI in male adult rats was induced by topical application of a stripe of filter paper saturated with 50% FeCl3 for a period of 10 min. Occlusion of the LAD resulted from the appearance of an adherent mixed thrombus within 30 minutes. On the ECG, elevated ST segments were observed following occlusion, characteristic of MI.
Results: Upon harvesting the heart, dual staining with Evans blue and triphenyltetrazolium chloride (TTC) revealed an infarct covering 33% of the left ventricular area provoked by the thrombotic occlusion. Animals treated with 30 mg/kg of acetyl salicylic acid (ASA) 30 min prior to FeCl3 application failed to generate the elevated ST-segment and no infarct area was observed. Cardiac troponin I, measured 24h post MI, increased from 0.4 to 343.4 ng/mL. Cardiac function evaluated 24h post MI using echocardiography calculated a 12 % reduction of the fractional shortening, 8% of the ejection fraction and 8.7% of the stroke volume while we observed a 9.3% increase in heart rate and a 24.8% increase in end systolic volume.
Conclusions: In conclusion, we present that this rat model of acute MI provoked by application of FeCl3 is a more clinically relevant model to be used in screening for new antithrombic or thrombolytic pharmaceutical treatments.
To cite this abstract in AMA style:Laurent C-, Dupré É, Demeule E, Salvail D. New Rat Model of Ferric Chloride-induced Acute Coronary Artery Thrombosis for Pharmaceutical Treatment of Myocardial Infarction [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/new-rat-model-of-ferric-chloride-induced-acute-coronary-artery-thrombosis-for-pharmaceutical-treatment-of-myocardial-infarction/. Accessed December 5, 2021.
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