Abstract Number: PB0381
Meeting: ISTH 2021 Congress
Background: Activated partial thromboplastin time (APTT) is widely used as a screening test for that characterizes coagulation of the blood. Recently, new ISTH lupus anticoagulant (LA) detection guidance described that ellagic acid activator reagent might show acceptable sensitivity in some reagents.
Aims: The purpose of this study was to perform the basic evaluation of newly developed APTT reagent (New-APTT), which uses ellagic acid and synthetic phospholipids, and investigate the LA sensitivity.
Methods: Four kinds of APTT reagents including New-APTT, current reagent with ellagic acid and two reagents prepared from silica activator were used. The within-run precision and the between-day precision were determined from 10 times measurements and 15 times measurements, five times a day over three days, in normal and abnormal controls, respectively. The comparative study against current ellagic acid reagent was performed using total 100 clinical samples including 37 LA-positive. LA sensitivity was evaluated among four APTT reagents in nine kinds of LA-positive controls by index of circulating anticoagulant (ICA) values calculated from the mixing tests.
Results: The coefficient of variation % (CV%) in within-run and between-day precision were within 0.77% and 1.59% in both normal and abnormal controls, respectively. The correlation coefficient and the regression equation were 0.899 and y=1.42x-14.26, respectively. The clotting times of New-APTT was more prolonged than those of current reagent in LA-positive and LA-positive with anticoagulant drug samples significantly. In LA sensitivity, the means of ICA values in New-APTT, current reagent and two silica reagents were 26.2, 26.7, 21.6 and 25.1, respectively. The significant difference was not observed among the reagents.
Conclusions: New-APTT showed an excellent analytical performance and had a high LA sensitivity comparable to silica reagents in LA-positive controls and clinical samples.
To cite this abstract in AMA style:Kumano O, Shimomura D, Kitano K, Arai N, TM. Newly Developed Ellagic Acid Based APTT Reagent Showed High Lupus Anticoagulant Sensitivity [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 2). https://abstracts.isth.org/abstract/newly-developed-ellagic-acid-based-aptt-reagent-showed-high-lupus-anticoagulant-sensitivity/. Accessed May 16, 2022.
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