Background: Thrombin Generation (TG) assay is a comprehensive test that assesses thrombin formation during the coagulation process.  ST-Genesia® is an automated system (STG, Diagnostica Stago) which provides a normalization of TG parameters against reference plasma in order to reduce technical variability.

Aims: To determine normal values for bleeding conditions with STG-BleedScreen® (STG-BLS) test in a healthy population.

Methods: We tested: 44 healthy individuals: 19 women and 25 men aged between 22 and 85 years.  STG-BLS test was performed with ST-Genesia® system. Parameters:  Lag time (ratio), peak high (%), time to peak (ratio), endogenous thrombin potential (ETP, %), velocity index (Vel %) and start tail (ratio) were analysed in absolute and normalized units.  Normal reference intervals for STG-BLS parameters were calculated for all individuals and by sex and age (<50 and ≥ 50 years).

Results: Table 1. Normalized Bleedscreen parameters
Table 1 shows all normalized parameters. Although the variables do not show a normal distribution, mean values were similar to median values. Normalized variables showed lower variability than absolute values. In women aged <50, higher values were observed for peak height and ETP (figure 1).

Figure 1. Peak height (blue) and ETP (red) by sex and age group.

Conclusions: We found differences in Bleedscreen parameters according age and sex, for this reason we consider to establish normal values, subgroups of age and sex have to be taken into account.

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ISTH Congress Abstracts - https://abstracts.isth.org/abstract/normal-values-in-thrombin-generation-parameters-in-hemorraghic-diseases/