Abstract Number: PB0149
Meeting: ISTH 2020 Congress
Background: Intracerebral hemorrhage (ICH) is the most feared complication of anticoagulant therapy, and haematoma expansion during new oral anticoagulants treatments is still under debate. To study this event in more detail and to test therapeutic strategies, experimental models of ICH have been developed.
Aims: In this work, we studied the effects of Warfarin and new oral anticoagulants (NOACs) (Rivaroxaban, Apixaban and Dabigatran) in haematoma expansion using a ICH model in Rats.
Methods: Oral anticoagulants were given by gavage at different doses and, at the peak of their anticoagulant and antithrombotic activities, ICH was induced by injecting collagenase VII-S into the left striatum. Haematoma lesion volume was determined 24h later using MRI and H&E-stained brain sections. We also estimated Blood Brain Barrier breakdown by Evans Blue staining and quantified the amount of hemoglobin in the brain using Drabkin’s reagent. Neurological deficits were evaluated using Body Elevated Swing Test. Differences between groups were analyzed using analysis of variance followed by ANOVA test with Origin 9.0 software.
Results: We observed that Rivaroxaban and Apixaban (18 mg/kg) or Dabigatran (9 mg/kg) did not expand lesion volume as Warfarin (0,5 and 1 mg/kg) when compared to control group, although Rivaroxaban showed a higher bleeding tendency than Apixaban and Dabigatran (Figures 1 and 2A). We observed a clear correlation between the images obtained by MRI and histological staining. These findings were confirmed by Evans blue staining and hemoglobin content using Drabkin’s reagent, which showed an increase in rats treated with warfarin but not with NOACs. We did not observe any difference in neurological test (Figure 2B).
Conclusions: Unlike Warfarin, NOACs do not promote larger haematoma volumes in a collagenase model of ICH in Rats.
To cite this abstract in AMA style:Ferreira J, Fonseca R, Coelho P, Rosado P, Mourao P. Novel Oral Anticoagulants Do Not Expand Haematoma Volume in a Intracerebral Hemorrhage Model in Rats [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/novel-oral-anticoagulants-do-not-expand-haematoma-volume-in-a-intracerebral-hemorrhage-model-in-rats/. Accessed December 5, 2021.
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