Abstract Number: PB0146
Meeting: ISTH 2022 Congress
Background: We recently demonstrated that platelets from severely obese patients show higher surface expression levels of GPVI and CLEC-2, and up-regulated signaling pathways compared to lean-matched controls, revealing that obesity induces an hyperactivation on platelets (Barrachina et al. ATVB; 2021;41:478–490).
Aims: To evaluate the impact of obesity in thrombus formation and platelet reactivity by using a novel ex vivo microfluidics assay on whole blood.
Methods: Severely obese patients (with no major comorbidities) and lean-matched controls were recruited. Whole-blood microfluidics assays evaluated the shear-dependent thrombus formation process over collagen-H in non-coagulating conditions. Platelet adhesion and activation parameters (integrin activation, P-selectin, and phosphatidylserine exposure) were recorded. Moreover, citrated blood was also perfused over a collagen surface to induce shear-dependent thrombus and fibrin formation, where tissue factor (TF) was partly included to induce activation of the extrinsic coagulation pathway promoting thrombin generation.
Results: In obese patients, increasing thrombus size and volume was observed on collagen. Adhesion parameters were slightly higher in obese patients whereas no major differences between groups were observed in activation parameters. Obese patients showed a slightly more rapid fibrin formation following adhesion to collagen in the presence of TF. In the latter conditions, there were also increased levels in coagulation parameters, such as fibrin formation and phosphatidylserine exposure, in blood from obese patients in comparison to the lean-matched subjects.
Conclusion(s): This is the first study evaluating the effect of obesity on ex vivo collagen-induced thrombus formation in flowing blood under both coagulating and non-coagulating conditions. Overall, we show that obesity enhances the platelet thrombotic phenotype to some extent. These results are in line with our previous studies on washed platelets and confirm an increased atherothrombotic risk in obese patients.
To cite this abstract in AMA style:Troitiño S, Fernández D, Hermida-Nogueira L, Buján-Garmendia S, López-Fernández D, Sueiro A, García Á. Obese patients show enhanced ex vivo collagen-induced thrombus formation in flowing human blood [abstract]. https://abstracts.isth.org/abstract/obese-patients-show-enhanced-ex-vivo-collagen-induced-thrombus-formation-in-flowing-human-blood/. Accessed March 4, 2024.
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