Abstract Number: VPB1279
Meeting: ISTH 2022 Congress
Theme: Platelet Disorders, von Willebrand Disease and Thrombotic Microangiopathies » Blood Cells and Vessel Wall
Background: Oxidative stress plays a major role in the progression of kidney disease. Nitric Oxide (NO), malondialdehyde (MDA), nitrotyrosine, annexin V, myeloperoxidase (MPO), non-esterified fatty acids (NEFA), fatty acid binding protein (FABP) and inflammatory cytokines are commonly used biomarkers of oxidative stress in various clinical conditions.
Aims: The purpose of this investigation is to compare the levels of circulating oxidative stress biomarkers including L-FABP in stage V chronic kidney disease (CKD-V) patients on hemodialysis (HD) compared to normal healthy controls.
Methods: Blood samples were collected in sodium citrate tubes from stable patients with CKD-V who are treated at Loyola University Medical Center Outpatient Dialysis Unit. Normal human plasma (NHP) samples was commercially obtained. Sandwich enzyme-linked immunosorbent assay (ELISA) methods were utilized to evaluate plasma levels of NO, MDA, nitrotyrosine, annexin V, MPO, troponin, VEGF, NEFA, plasminogen activator inhibitor 1 (PAI-1),inflammatory cytokines and L-FABP.
Results: The CKD-V cohort showed a statistically significant elevation in the levels of annexin V (7.57 ± 5.24 ng/mL) NO (34.74 ± 19.74 pg/mL), and L-FABP (106,599 ± 176,729 pg/mL) compared to the NHP group. Lower levels of MPO (100.2 ± 19.28 mU/mL) and NEFA (0.56 ± 0.34 mEq/L) was observed in comparison to the NHP group (p < 0.05). L-FABP levels were significantly elevated, almost 20-fold higher in CKD-V patients compared to the NHP group. Spearman correlation revealed a negative correlation of L-FABP with NEFA ( r= -0.22; p < 0.05) and a positive correlation of L-FABP with nitrotyrosine (r = 0.36; p < 0.05) in the CKDV cohort.
Conclusion(s): These results suggest that as oxidative stress increases in chronic kidney disease patients, the rate of lipid peroxidation also increases, thereby converting NEFAs into lipoperoxides. As levels of these lipoperoxides increase, L-FABP levels also increase as a protective measure to excrete the toxic byproducts out of the body
To cite this abstract in AMA style:
Sridharan D, Bansal V, Siddiqui F, Karumanchi P, Odeh A, Iqbal O, Kantarcioglu B, Fareed J. Oxidative Stress Biomarkers and Their relevance to fatty Acid-Binding Protein in Stage-V Chronic Kidney Disease Patients on Hemodialysis [abstract]. https://abstracts.isth.org/abstract/oxidative-stress-biomarkers-and-their-relevance-to-fatty-acid-binding-protein-in-stage-v-chronic-kidney-disease-patients-on-hemodialysis/. Accessed March 22, 2024.« Back to ISTH 2022 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/oxidative-stress-biomarkers-and-their-relevance-to-fatty-acid-binding-protein-in-stage-v-chronic-kidney-disease-patients-on-hemodialysis/