Abstract Number: PB0912
Meeting: ISTH 2021 Congress
Theme: Platelet Disorders, von Willebrand Disease and Thrombotic Microangiopathies » von Willebrand Factor Biology
Background: Palmitic acid is one of the most abundant saturated fatty acid in the plasma of obese patients. Obesity which finally leads to the insulin resistance and type 2 diabetes results in a dysfunction of vascular endothelium and proinflammatory response. In human with type 2 diabetes or insulin resistance higher level of secreted von Willebrand Factor (vWF) is associated with an elevated risk of cardiovascular disease.
Aims: The aim of the study was to test whether in vitro palmitate treatment of Human Umbilical Vein Endothelial Cells (HUVEC) affects gene expression, secretion and protein level of vWF.
Methods: HUVECs were treated with palmitate complexed with BSA or BSA alone as a control. TaqMan RT‑qPCR was performed to examine vWF, P-selectin, CD63 genes expression in the control and palmitate-treated cells. HUVECs also were treated with histamine and forskolin to compare using ELISA test a basal and stimulated secretion of vWF. Western Blot was employed to analyze vWF protein level in cell lysates. One-way ANOVA statistical analysis was performed.
Results: Incubation of HUVECs with palmitate (100 μM) resulted in the increased vWF gene expression in comparison to BSA exposed controls after 24h of treatment, while P-selectin and CD63 transcripts level remained unchanged. Moreover, 48h treatment of the cells with palmitate increased histamine- and forskolin-stimulated secretion of VWF, without influencing the basal secretion. Furthermore, the level of the mature protein form of vWF was elevated in the palmitate-treated cells.
Conclusions: In vitro incubation of HUVECs with palmitate increases vWF gene expression and protein vWF. Moreover it increases histamine- and forskolin stimulated secretion of vWF. These results suggest a potential link between obesity and dysfunction of vascular endothelium.
This work was supported by a grant number 2016/23/B/NZ3/03116 from the National Science Center, Poland, and by statutory funds from the Nencki Institute of Experimental Biology.
To cite this abstract in AMA style:
Seliga AK, Zabłocki K, Bandorowicz-Pikuła J. Palmitate Increases Gene Expression and Stimulated Secretion of von Willebrand Factor in Human Umbilical Vein Endothelial Cells [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 1). https://abstracts.isth.org/abstract/palmitate-increases-gene-expression-and-stimulated-secretion-of-von-willebrand-factor-in-human-umbilical-vein-endothelial-cells/. Accessed September 24, 2021.« Back to ISTH 2021 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/palmitate-increases-gene-expression-and-stimulated-secretion-of-von-willebrand-factor-in-human-umbilical-vein-endothelial-cells/