Abstract Number: PB1136
Meeting: ISTH 2021 Congress
Background: Cancer-associated venous thromboembolism (VTE) increases mortality and morbidity. However, limited tools are available to identify high risk patients. microRNAs (miRNAs) are small non-coding RNAs that regulate protein expression. miRNAs seem to regulate cancer progression and VTE. We have previously reported a profile of 7 plasma miRNAs able of estimate the risk of future VTE at diagnosis in biliopancreatic cancer patients (AUC = 0.95; 95% Confidence Interval [0.987, 1]) (1)
Aims: To develop an open online app to rapidly calculate the risk of future VTE events in biliopancreatic cancer patients using a plasma profile of 7 miRNAs.
Methods: The interface was created with using R (v4.0.2) and Shiny (v1.4). We created a short introduction followed by a friendly menu where any researcher can upload the expression level of 7 miRNAs in their patients and obtain a VTE prediction value.
Results: In this tool, any researcher worldwide can introduce the miRNA expression data from their biliopancreatic cancer patients and rapidly obtain a prediction of future VTE events. The steps in the process are:
1) Quantify by RT-qPCR the expression levels of 7 miRNAs (miR-486-5p, miR-106b-5p, let-7i-5p, let-7g-5p, miR-144-3p, miR-19a-3p and miR-103a-3p) and the normalizer (miR-93-5p).
2) Upload raw Ct values in a user-friendly interface.
3) Download a file with the predictions for each study subject.
Conclusions: We have developed the Shiny App Pancreatic-miRaCan-VTE, a free available online tool to easy and rapidly estimate the risk of future VTE events in biliopancreatic cancer patients. The use of Pancreatic-miRaCan-VTE could support the personalized management of biliopancreatic cancer patients in whom to tailor thromboprophylaxis to prevent adverse thrombotic complications. ISCIII-FEDER (PI17/00495, PI20/00075), GVA (ACIF/2017/138), Sociedad Española de Trombosis y Hemostasia.
1. Oto J et al. Int J Mol Sci 2020 Jan 28;21(3):840. doi: 10.3390/ijms21030840.
To cite this abstract in AMA style:Oto J, C Larsen A, Plana E, Herranz R, Cana F, Hervás D, Cañada A, Fernández-Pardo Á, R Kristensen S, Thorlacius-Ussing O, Medina P. Pancreatic-miRaCan-VTE: A R Shiny App to Aid in the Diagnosis of Pancreatic Cancer-associated VTE [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 2). https://abstracts.isth.org/abstract/pancreatic-miracan-vte-a-r-shiny-app-to-aid-in-the-diagnosis-of-pancreatic-cancer-associated-vte/. Accessed September 30, 2023.
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