Abstract Number: PB1010
Meeting: ISTH 2021 Congress
Background: The safety of platelet concentrates (PC) is of central importance in transfusion medicine, and is mainly guaranteed by the selection of suitable donors, the testing of known infectious agents, and a limitation of the storage time as part of the manufacturing process. Given the emergence and spread of novel transmissible infectious agents, the importance of pathogen reduction procedures of PC has increased significantly in recent years.
Aims: To dissect alterations in platelet function and integrity in pathogen-reduced PC prepared by three different procedures.
Methods: The study design is based on a pool-and-split approach. Platelet products treated with Amotosalen/UVA, Riboflavin/UV, and UVC as well as untreated platelet products are prepared from pooled plasma-reduced PC and stored under standard conditions. Platelet products are tested for functional parameters including platelet aggregation, and P-selectin- and phosphatidylserine expression. Mitochondrial membrane potential as a marker of apoptosis is assessed by TMRE (tetramethylrhodamine, ethyl ester) sequestration. Moreover, alterations in adhesive properties and platelet stability are investigated by the application of wall shear stress in collagen-coated micro-fluidic channels and after cold treatment at 4°C.
Results: Our study protocol is designed to examine platelet function after application of three common pathogen reduction procedures compared with untreated controls. The pool-and-split method enables alterations in platelet function to be estimated with little impact of the individual donor-associated variations. Furthermore, our test concept provides for a shear stress challenge on platelets that mimics the physiological situation in the vascular system and could reveal latent defects.
Conclusions: In view of the increasing demands on the infection safety of platelet concentrates, parallel testing and application of challenging stress conditions is a valuable approach for the evaluation of novel manufacturing procedures and their effect on platelet functionality.
To cite this abstract in AMA style:Visser M, Schwarz W, Rausch-Mueller A, Volpe S, Hilger A, Salge-Bartels U. Pathogen-reduced Platelet Concentrates: Novel Concepts to Elucidate the Impact of Different Technologies on Platelet Function [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 2). https://abstracts.isth.org/abstract/pathogen-reduced-platelet-concentrates-novel-concepts-to-elucidate-the-impact-of-different-technologies-on-platelet-function/. Accessed February 28, 2024.
« Back to ISTH 2021 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/pathogen-reduced-platelet-concentrates-novel-concepts-to-elucidate-the-impact-of-different-technologies-on-platelet-function/