Abstract Number: PB0840
Meeting: ISTH 2021 Congress
Theme: Platelet Disorders, von Willebrand Disease and Thrombotic Microangiopathies » ADAMTS13 and TTP
Background: Currently, the only known role of ADAMTS13 is in hemostasis, in its proteolytic activity to its only known substrate VWF. Having only one substrate, and one role, is unlikely as other ADAMTS proteases have multiple substrates and/or roles. Few studies tried to explore additional roles for ADAMTS13 outside of hemostasis and have eluted to that possibility.
Aims: However, it is unclear whether these roles are directly dependent on the activity of ADAMTS13 or they are a result of the size of VWF multimers distribution.
Methods: Here, we have adapted the novel technique BioID to be used in-vitro in plasma, to map the interactome of ADAMTS13.
Results: Our yield was an observation of 199 unique proteins, 108 of which were significant, and 6 of which are extracellular and of interest, including the auto-labelling of ADAMTS13, VWF and potential novel interactions with vitronectin or plasminogen. Validation studies using surface plasmon resonance (SPR) disregarded the interaction with vitronectin, and confirmed the interaction with plasminogen.
Conclusions: Further analysis using the SPR eluded to the possible interaction of ADAMTS13 to Kringle-domains of plasminogen, but not to proteins like prothrombin.
To cite this abstract in AMA style:
Madarati H, Singh K, Sparring T, Kretz C. Plasma BioID of ADAMTS13 [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 2). https://abstracts.isth.org/abstract/plasma-bioid-of-adamts13/. Accessed November 28, 2021.« Back to ISTH 2021 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/plasma-bioid-of-adamts13/