Abstract Number: PB0372
Meeting: ISTH 2020 Congress
Background: Microvesicles (MVs) are small double-membrane encapsulated particles shed from activated cells. High expression levels of negatively charged phospholipids, and in particular phosphatidylserine (PS), contribute to the procoagulant properties of microvesicles. Several case-control studies have shown elevated plasma levels of platelets-derived MVs (PDMVs) in patients with venous thromboembolism (VTE) compared to control subjects. As PDMVs is an acute phase reactant, it is not known whether PDMVs are a risk factor or merely a consequence of the acute VTE event.
Aims: To investigate the association between plasma levels of PDMVs and future risk of incident VTE.
Methods: A nested case-control study including 264 VTE cases and 601 randomly selected age- and sex-matched controls was derived from a population-based cohort (the Tromsø study). Plasma samples were collected at baseline and MVs were isolated by ultracentrifugation, stained for expression of PS and CD41a/isotype-control, and further analyzed by flow cytometry. Odds ratio (OR) with 95% confidence interval (CI) were calculated for VTE risk across quartiles of the proportion of PDMPs in control subjects.
Results: The plasma concentration of PS-positive MVs increased with increasing quartiles of PDMVs, as well as risk of incident VTE. Participants with PDMVs in the highest quartile had an OR of 1.61 (95% CI 1.07-2.44) for VTE compared to those in the lowest quartile. The OR was unchanged after adjustment for age, sex, body mass index, and CRP. The effect was most apparent for unprovoked deep vein thrombosis (DVT) (OR 2.35, 95% CI 0.92-6.78). A substantially higher risk for VTE was observed in samples taken shortly before the VTE event.
Conclusions: High levels of PDMPs were associated with future risk of incident VTE, suggesting that high plasma levels of PDMVs are not only a consequence of an acute thrombotic event, but also a risk marker of future VTE.
To cite this abstract in AMA style:Snir O, Wilsgård L, Latysheva N, Wahlund C, Hindberg K, Hansen J-. Plasma Levels of Platelet-derived Microvesicles Are Associated with Future Risk of Venous Thromboembolism [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/plasma-levels-of-platelet-derived-microvesicles-are-associated-with-future-risk-of-venous-thromboembolism/. Accessed January 21, 2022.
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