Abstract Number: PB0134
Meeting: ISTH 2020 Congress
Background: Obesity is one of the main health problems in industrialized countries. The contribution of multiple factors developed in obesity can hardly be modeled in vitro. In this context, the development of animal models mimicking human obesity could be essential.
Aims: The main goal of the present study was to standardize a blood collection and platelet isolation methodology to study a diet-induced obesity (DIO) rat model both at biochemical and functional level. Moreover, the results obtained were compared between DIO and control groups in order to understand platelet dysfunction in obesity.
Methods: According to the methodology, optimal blood collection was obtained from vena cava using a 19G needle and a 10mL syringe obtaining up to 8 mL. Platelet isolation was based on a serial of centrifugations avoiding platelet activation.
Results: Our results show that the DIO model simulate perfectly the pathological mechanistic of obesity since weight gain and fasting glucose are increased in obese rats. Moreover, the methodology showed a greater grade of reproducibility between groups, and pointed towards an increment in platelet counts in the DIO group, in line with human studies. To further understand platelet dysfunction in obesity, we demonstrated that platelet adhesion to collagen and the platelet levels of the active form of Src (pTyr419) were increased in the DIO rat model. These results pointed towards a potential dysfunction on GPVI and Src family kinases (SFK)-related signalling pathways in obesity.
Conclusions: These results confirm that obesity, in line with human studies, present an atherothrombotic risk that should be considered, and highlight the relevance of considering novel antithrombotic drug targets in these patients, such as GPVI.
To cite this abstract in AMA style:Barrachina MN, Morán LA, Izquierdo I, Casanueva FF, Pardo M, García Á. Platelet Analysis of Diet-Induced Obesity Rat Model: Elucidating Platelet Dysfunction in Obesity [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/platelet-analysis-of-diet-induced-obesity-rat-model-elucidating-platelet-dysfunction-in-obesity/. Accessed February 21, 2024.
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