Abstract Number: PB1846
Meeting: ISTH 2020 Congress
Background: Transfusion-related acute lung injury (TRALI) is a severe pulmonary reaction due to blood transfusions. The pathophysiology of this complication is still not widely elucidated by the scientific community, especially regarding the direct role of blood platelets within the cellular mechanism responsible for the development of TRALI.
Aims: Beside the recently described proinflammatory functions of platelet, the conflicting reports of platelets involvement in TRALI have led us to investigate their role in its onset. In order to address this question, we developed a two-hit mouse model of TRALI induced by injections of lipopolysaccharide (LPS) and anti-MHC I antibody, which is the equivalent of HLA Class I in Human.
Methods: In this study, a mouse model was used to induce the development of antibody-mediated acute lung injury (ALI) through injections of lipopolysaccharide (LPS) and an anti-MHC class I antibody. BALB/c mice were pretreated with an anti-GPIbα antibody, which induces platelet depletion, or ML354, a PAR-4 receptor pathway inhibitor, 30 minutes prior to TRALI induction.
Results: Depletion of platelets prior to TRALI induction appeared to reduce the severity of TRALI without completely inhibiting its development. Also, inhibition of platelet activation by ML354 did not prevent the onset of TRALI. Finally, the stimuli used of TRALI induction also triggered specific platelet activation upon ex vivo stimulation.
Conclusions: This study suggests that blood platelets are not critically required for TRALI induction although they are to some extent involved in its pathophysiology.
To cite this abstract in AMA style:Cognasse F, Tariket S, Hamzeh-Cognasse H, Arthaud C-, Eyraud M-, Bourlet T, Berthelot P, Laradi S, Fauteux-Daniel S, Garraud O. Platelet Depletion Limits the Severity But Does Not Prevent the Occurrence of Experimental Transfusion-related Acute Lung Injury [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/platelet-depletion-limits-the-severity-but-does-not-prevent-the-occurrence-of-experimental-transfusion-related-acute-lung-injury/. Accessed May 6, 2021.
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