Abstract Number: LPB0074
Meeting: ISTH 2021 Congress
Background: The specific role of platelets in the course of sepsis is not yet fully understood, probably related to the paradox of platelets being potentially beneficial but also deleterious via their thrombotic functions.
Aims: To evaluate the impact of experimental thrombocytopenia on the pathogenesis of polymicrobial-induced septic shock in mice and to investigate whether transfusion of platelets, either fully functional or with impaired hemostatic properties, might have beneficial effects.
Methods: Septic shock was induced by cecal ligation and puncture (CLP); sham-operated animals were used as control. Experimental depletion of circulating platelets was induced by intravenous injection of a rat anti-mouse GPIbα monoclonal antibody. Transfusion of either native platelets, platelets treated with the antiplatelet drugs acetylsalicylic acid (ASA) and clopidogrel, or GPIIbIIIa-deficient platelets treated with clopidogrel and ASA was performed 4 h after CLP surgery.
Results: Experimental depletion of circulating platelets negatively affected the pathogenesis of septic shock in mice, worsening systemic inflammation, coagulopathy, organ damage and mortality, raising the question of whether a higher platelet count could protect against septic shock. Transfusion of either fully functional platelets or platelets with combined treatment with ASA and clopidogrel, with or without additional GPIIbIIIa deficiency, 4 h after CLP surgery, afforded an immediate return of circulating platelet counts to their initial values before surgery. However, transfusion of each of the three types of platelets did not prevent arterial hypotension, inflammatory response, coagulopathy and organ damage during septic shock.
Conclusions: Experimental depletion of circulating platelets negatively affects polymicrobial septic shock, while platelet transfusion has no significant beneficial effect. Transfusion of platelets may nevertheless be safely used to treat profound thrombocytopenia associated with bleeding during sepsis, without having any deleterious impact by fueling sepsis.
To cite this abstract in AMA style:Rabouel Y, Magnenat S, Lefebvre F, Delabranche X, Gachet C, Hechler B. Platelet Transfusion Does Not Prevent Experimental Polymicrobial-induced Septic Shock in Mice [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 2). https://abstracts.isth.org/abstract/platelet-transfusion-does-not-prevent-experimental-polymicrobial-induced-septic-shock-in-mice/. Accessed February 28, 2024.
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