Abstract Number: PB0319
Meeting: ISTH 2021 Congress
Theme: Diagnostics and OMICs » Blood Components and Management
Background: The diversion of the first flow of blood into a pouch has been widely adopted in blood donation systems as a means to reduce whole-blood unit contamination from skin bacteria. Analyses of platelet biology require careful control of variables, including collection methods. The diversion pouch may serve as an alternative blood collection method to standard venipuncture, allowing a greater number of healthy blood bank donors to serve as research subjects.
Aims: To determine if the functional and bioenergetic profiles of platelets isolated from the diversion pouch are representative of platelets isolated from venipuncture.
Methods: Whole blood from the diversion pouch was collected into citrated tubes within two minutes of collection. Platelets were isolated and washed following standard protocols. Platelet activated fibrinogen binding site was determined at baseline and following activation (thrombin [0.075 IU/ml] and ADP [1.25uM]) with PAC-1 antibodies. The bioenergetic profile (aerobic and anaerobic) of washed platelets was determined by the 24-well format Seahorse extracellular flux analyzer.
Results: Platelets isolated from venipuncture and diversion pouch exhibit similar PAC-1 expression at baseline and with activation by thrombin and ADP (Figure 1). No differences in the bioenergetic profile were noted between both groups (Figure 2).
Platelet functional profile of whole blood samples obtained by venipuncture and diversion pouch are similar.
Platelet bioenergetic profile of whole blood samples obtained by venipuncture and diversion pouch are similar.
Conclusions: Platelets isolated from the diversion pouch exhibit similar functional and bioenergetic profiles when compared to the standard venipuncture method. These findings suggest that platelets isolated from the diversion pouch are a suitable option for functional and bioenergetic testing analyses.
To cite this abstract in AMA style:
Esparza O, Hernandez G, Kelher M, Kelly K, Silliman C, Dumont L, Davizon-Castillo P. Platelets Collected from Blood Diversion Pouches Are an Appropriate Alternative for Functional and Bioenergetic Analyses [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 2). https://abstracts.isth.org/abstract/platelets-collected-from-blood-diversion-pouches-are-an-appropriate-alternative-for-functional-and-bioenergetic-analyses/. Accessed September 21, 2023.« Back to ISTH 2021 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/platelets-collected-from-blood-diversion-pouches-are-an-appropriate-alternative-for-functional-and-bioenergetic-analyses/