Abstract Number: PB0201
Meeting: ISTH 2021 Congress
Background: Coronavirus diesease-2019 (COVID-19) is associated with increased thromboembolic complications. COVID-19 related coagulotpathy (CAC) involves derangements in both cellular and plasma elements of the coagulation system. We found that COVID19 infection is accompanied with the development of procoagulant platelet phenotype and an increased resistance to clot lysis. As more people recover from COVID-19 infection but still develop thrombotic complications, discussion has begun over the possibility of persistent CAC even after the acute infection period.
Aims: To investigate the platelet activation after remission of COVID-19 infection.
Methods: We included plasma donors who had a PCR confirmed mild COVID-19 infection within the last 6 months (exCOVID-19 Group) in the study. A control group consisted of donors who did not have COVID-19. We collected citrate blood from study participant and investigated CD62p expression, Annexin V and GPIV shedding with flow cytometry. All participants gave informed consent and institutional ethics committee approved the study protocol. We compared the data with Mann-Whitney test using GraphPad Prism.
Results: Twenty-four convalescent plasma donors (11 Male, 13 Female) with a history of COVID-19 infection were included. Mean age (SD) was 39 ± 13 years. Median duration (interquartile range) after acute COVID-19 infection was 82 days (73-106). Baseline GPIV shedding rate was higher in exCOVID-19 group (1 ± 0 vs. 1.13 ± 0.06). However, platelet response to collagen-related peptide (CRP) did not differ between both groups. Baseline CD62p expression and CD62p expression after activation with thrombin receptor associated protein (TRAP) was similar between exCOVID-19 group and control group. The percentage of CD62p and Annexin V double positive platelets was similar between groups.
Conclusions: Our findings suggest that procoagulant platelet phenotype is not present after mild COVID-19. Further studies should investigate the alterations in plasma components of coagulation and fibrinolytic system after COVID-19.
To cite this abstract in AMA style:Uzun G, Pelzl L, Abou-Khalel W, Singh A, Nowak-Harnau S, Althaus K, Bakchoul T. Platelets from Donors of Convalescent Plasma after Mild COVID-19 Do Not Express Procoagulant Phenotype [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 2). https://abstracts.isth.org/abstract/platelets-from-donors-of-convalescent-plasma-after-mild-covid-19-do-not-express-procoagulant-phenotype/. Accessed October 1, 2023.
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