Abstract Number: PB0192
Meeting: ISTH 2020 Congress
Theme: Coagulation and Natural Anticoagulants » Coagulation Factors and Inhibitors
Background: Point-of-Care and storage-stable, single-use 8-channel microfluidic chip for rapid testing of DOACs and DOAC reversal agents under whole blood flow.
Aims: Develop a rapid POC detection of DOAC function and DOAC reversal using whole blood.
Methods: We designed an injection-molded 1”x3” device with 8-parallel flow paths crossing over a bioprinted surface of collagen/tissue factor. The device is rapidly primed and compatible with manual or automated pipetting as well as operates under venous or arterial shear rates using constant flowrate or constant pressure-drop modes. Platelet and fibrin deposition were monitored dynamically by small benchtop chip reader.
Results: For whole blood clotting at wall shear rate of 200 s-1 for 500 sec, the intrachip CV for platelet and fibrin deposition was 20% (across 8 lanes) and the interchip CV was 25% for platelet and 30% for fibrin deposition (across 10 healthy male and female donors). No significant differences in platelet or fibrin deposition were detected for a blood sample tested on a freshly made chip or a chip stored dry for 6 months. Using the dynamic fibrin signal, dose-response testing of flowing whole blood resulted in an IC50 = 120 nM for the direct oral anticoagulant rivaroxaban (Factor Xa inhibitor). In an ex vivo test for DOAC reversal in whole blood (200 s-1), a single chip test detected within 300 sec the potent reduction in fibrin deposition due to 200 nM rivaroxaban and the complete reversal of rivaroxaban by a 1:1 dose of Andexxa back to the original baseline fibrin deposition (no rivaroxaban).
Conclusions: We demonstrate the ability to manufacture single-use and storage-stable 8-channel chips that operate automatically for rapid detection of DOAC potency and DOAC reversal.
[Point of Care chip for whole blood testing under flow (A). Detection of DOAC reversal (B).]
To cite this abstract in AMA style:
Rossi J, Diamond S. Point-of-Care and Storage-Stable, Single-Use 8-Channel Microfluidic Chip for Rapid Testing of DOACs and DOAC Reversal Agents under Whole Blood Flow [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/point-of-care-and-storage-stable-single-use-8-channel-microfluidic-chip-for-rapid-testing-of-doacs-and-doac-reversal-agents-under-whole-blood-flow/. Accessed September 27, 2023.« Back to ISTH 2020 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/point-of-care-and-storage-stable-single-use-8-channel-microfluidic-chip-for-rapid-testing-of-doacs-and-doac-reversal-agents-under-whole-blood-flow/