Abstract Number: PB1367
Meeting: ISTH 2020 Congress
Background: Immune thrombocytopenic purpura (ITP) is an autoimmune disorder. Besides platelets specific auto-antibodies the T-cells imbalance may contribute in occurrence of this disease. This imbalance may be influenced by polymorphism of glutathione S transferases as observed in other autoimmune diseases.
Aims: Current study was planned to evaluate the impact of polymorphisms of glutathione S transferases on T-cells disparity in ITP patients and its association with disease severity.
Methods: Seventy six, confirmed ITP patients and 76 healthy controls were enrolled in this study following declaration of Helsinki. Peripheral blood was collected and complete blood profile parameters were recorded using XN 1000. The helper (Th) and cytotoxic (Tc) cells were enumerated by flow-cytometric analysis on BD FACS Calibur. GSTM1 and GSTT1 genes polymorphism were detected by multiplex PCR with beta-globin gene as internal control. The significant difference between newly diagnosed/persistent ITP or chronic ITP and healthy control groups was determined by F-test using SPSS ver. 21.
Results: Significantly low hemoglobin levels, platelets count with high immature platelet fraction were detected in newly diagnosed/persistent and chronic/refractory ITP patients (p= < 0.05). GSTM1 deletion was the most frequent genotype observed (45.45%). The patients with normal GST genotypes had an average Th/Tc ratio of 1.4 with less frequent bleeding symptoms while those with homozygous deletion of both GSTM1 and GSTT1 had a significantly low Th/Tc ratio of 0.8 with frequently observed gum bleeding.
Conclusions: Higher Th counts in patients with normal GST genotype may have protective effect as expressed by lower bleeding tendency as compared to patients with dual GSTM1 and GSTT1 null deletion. The dual deletion may have possible detrimental effect on immune function and antioxidant status suggesting a potential benefit of presence of functional GSTM1 and GSTT1 enzymes in ITP patients.
To cite this abstract in AMA style:Mukry SN, Shahni A, Arshad A, Shamsi TS. Possible Impact of Polymorphism of GSTM1 and GSTT1 Genes on Bleeding Tendency in Immune Thrombocytopenia [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/possible-impact-of-polymorphism-of-gstm1-and-gstt1-genes-on-bleeding-tendency-in-immune-thrombocytopenia/. Accessed October 2, 2023.
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