Abstract Number: PB1300
Meeting: ISTH 2021 Congress
Background: The levels of pro-inflammatory (e.g. leukotriene B4-LTB4) and pro-resolving (e.g. lipoxin A4-LXA4, and resolvin D1-RvD1) lipid mediators have been recently investigated in preeclampsia (PE). However, the production of these mediators throughout gestation in both healthy and diseased contexts remains unclear.
Aims: Investigate LTB4, LXA4, and RvD1 levels throughout gestation in pregnant women with risk factors for PE who either developed (N=11) or did not develop (N=7) the disease.
Methods: The ethics committee of the Federal University of Minas Gerais (#0618.0.203.000-10) approved the study protocol and all participants provided written informed consent. LTB4, LXA4 and RvD1 plasma levels were measured by immunoassays at three timepoints: 12-19, 20-29, and 30-34 weeks of gestation in both groups.
Results: Table 1 shows patients’ clinical characteristics.
|Parameters||Pregnant women who did not develop PE (N=17)||Pregnant women who developed PE (N=11)||P|
|Age (years)a||27 ± 8||27 ± 4||0.598|
|BMI at study admission (kg/m2)b||23.6 (23.2 – 25.9)||28.7 (22.5 – 41.8)||0.073|
|Number of gestationsb||1.0 (1.0 – 2.5)||2.0 (1.0 – 3.0)||0.235|
|SBP at study admission (mmHg)a||112 ± 11||119 ± 7||0.271|
|DBP at study admission (mmHg)a||71 ± 9||77 ± 8||0.138|
|2nd SBP measurement (mmHg)a||114 ± 9||116 ± 15||0.313|
|2nd DBP measurement (mmHg)a||71 ± 4||78 ± 13||0.072|
|SBP before delivery (mmHg)b||115 (110 – 120)||140 (135 – 171)||<0.001*|
|DBP before delivery (mmHg)b||70 (70 – 78)||110 (89 – 115)||<0.001*|
|Abbreviations: BMI, body mass index; SBP, systolic blood pressure; DBP, diastolic blood pressure; PE, preeclampsia. aStudent’s t-test. Parametric data are presented as mean ± standard deviation (SD). bMann-Whitney U-test. Non-parametric data are presented as median (25th – 75th percentiles). *P<0.05 = statistically significant.|
There was a trend of upregulated LTB4 levels throughout gestation in women who developed PE, but this difference was significant only at 30-34 weeks (Fig.1A). LXA4 levels showed a similar pattern with no difference between groups in any gestational age (Fig. 1B). Pregnant women who developed PE had lower RvD1 levels (Fig. 1C) and a decreased RvD1/LTB4 ratio (Fig. 1E) at 30-34 weeks compared to normotensive pregnant women (Norm). Contrarily, RvD1 levels were decreased in normotensive pregnant women at 12-19 weeks (Fig. 1C). LXA4 and RvD1 levels were higher at 30-34 weeks compared to 20-29 weeks in both groups (Fig. 1B and 1C, respectively). The LXA4/LTB4 ratio did not differ between groups in any gestational age evaluated, but it was higher at 30-34 weeks compared to 20-29 and 12-19 weeks of gestation in both groups (Fig. 1D). There was an interaction between the gestational outcome (preeclamptic vs. normotensive pregnancy) and the gestational age only for RvD1.
Conclusions: Imbalanced levels of LTB4, LXA4, and RvD1 may be associated with the excessive systemic inflammation that underlies PE pathogenesis.
Financial support: CNPq, FAPEMIG, CAPES and UFOP/PROPP.
To cite this abstract in AMA style:Perucci L, Pinto K, Silva S, Lage E, Teixeira P, Barbosa A, Alpoim P, Sousa L, Dusse L, Talvani A. Preeclampsia and Inflammatory Lipid Mediators: A Longitudinal Study [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 2). https://abstracts.isth.org/abstract/preeclampsia-and-inflammatory-lipid-mediators-a-longitudinal-study/. Accessed January 23, 2022.
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