Abstract Number: PB1300
Meeting: ISTH 2021 Congress
Background: The levels of pro-inflammatory (e.g. leukotriene B4-LTB4) and pro-resolving (e.g. lipoxin A4-LXA4, and resolvin D1-RvD1) lipid mediators have been recently investigated in preeclampsia (PE). However, the production of these mediators throughout gestation in both healthy and diseased contexts remains unclear.
Aims: Investigate LTB4, LXA4, and RvD1 levels throughout gestation in pregnant women with risk factors for PE who either developed (N=11) or did not develop (N=7) the disease.
Methods: The ethics committee of the Federal University of Minas Gerais (#0618.0.203.000-10) approved the study protocol and all participants provided written informed consent. LTB4, LXA4 and RvD1 plasma levels were measured by immunoassays at three timepoints: 12-19, 20-29, and 30-34 weeks of gestation in both groups.
Results: Table 1 shows patients’ clinical characteristics.
| Parameters | Pregnant women who did not develop PE (N=17) | Pregnant women who developed PE (N=11) | P |
| Age (years)a | 27 ± 8 | 27 ± 4 | 0.598 |
| BMI at study admission (kg/m2)b | 23.6 (23.2 – 25.9) | 28.7 (22.5 – 41.8) | 0.073 |
| Number of gestationsb | 1.0 (1.0 – 2.5) | 2.0 (1.0 – 3.0) | 0.235 |
| SBP at study admission (mmHg)a | 112 ± 11 | 119 ± 7 | 0.271 |
| DBP at study admission (mmHg)a | 71 ± 9 | 77 ± 8 | 0.138 |
| 2nd SBP measurement (mmHg)a | 114 ± 9 | 116 ± 15 | 0.313 |
| 2nd DBP measurement (mmHg)a | 71 ± 4 | 78 ± 13 | 0.072 |
| SBP before delivery (mmHg)b | 115 (110 – 120) | 140 (135 – 171) | <0.001* |
| DBP before delivery (mmHg)b | 70 (70 – 78) | 110 (89 – 115) | <0.001* |
| Abbreviations: BMI, body mass index; SBP, systolic blood pressure; DBP, diastolic blood pressure; PE, preeclampsia. aStudent’s t-test. Parametric data are presented as mean ± standard deviation (SD). bMann-Whitney U-test. Non-parametric data are presented as median (25th – 75th percentiles). *P<0.05 = statistically significant. | |||
Clinical characteristics of the studied pregnant women.
There was a trend of upregulated LTB4 levels throughout gestation in women who developed PE, but this difference was significant only at 30-34 weeks (Fig.1A). LXA4 levels showed a similar pattern with no difference between groups in any gestational age (Fig. 1B). Pregnant women who developed PE had lower RvD1 levels (Fig. 1C) and a decreased RvD1/LTB4 ratio (Fig. 1E) at 30-34 weeks compared to normotensive pregnant women (Norm). Contrarily, RvD1 levels were decreased in normotensive pregnant women at 12-19 weeks (Fig. 1C). LXA4 and RvD1 levels were higher at 30-34 weeks compared to 20-29 weeks in both groups (Fig. 1B and 1C, respectively). The LXA4/LTB4 ratio did not differ between groups in any gestational age evaluated, but it was higher at 30-34 weeks compared to 20-29 and 12-19 weeks of gestation in both groups (Fig. 1D). There was an interaction between the gestational outcome (preeclamptic vs. normotensive pregnancy) and the gestational age only for RvD1.
Plasma levels of inflammatory lipid mediators, and the ratios between pro-resolving and pro-inflammatory lipid mediators throughout preeclamptic and normotensive pregnancies.
Conclusions: Imbalanced levels of LTB4, LXA4, and RvD1 may be associated with the excessive systemic inflammation that underlies PE pathogenesis.
Financial support: CNPq, FAPEMIG, CAPES and UFOP/PROPP.
To cite this abstract in AMA style:
Perucci L, Pinto K, Silva S, Lage E, Teixeira P, Barbosa A, Alpoim P, Sousa L, Dusse L, Talvani A. Preeclampsia and Inflammatory Lipid Mediators: A Longitudinal Study [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 2). https://abstracts.isth.org/abstract/preeclampsia-and-inflammatory-lipid-mediators-a-longitudinal-study/. Accessed November 30, 2023.« Back to ISTH 2021 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/preeclampsia-and-inflammatory-lipid-mediators-a-longitudinal-study/