Abstract Number: PB1991
Meeting: ISTH 2020 Congress
Theme: Vascular Biology » Inflammation and Sepsis
Background: Erysipelas is an actual problem of health care. It is characterized by consistently high morbidity, increased tendency to recur, reduced the life quality and took important place in the structure of temporary disability. Erysipelas causes disorders of haemostasis and development of immune response. Activation of the macrophage system in the skin is accompanied by the expression of tissue factor, which leads to hypercoagulation in erysipelas.
Aims: The aim was to study the genetic polymorphism of TF (A603G, C1322T, C1812T, G1442C), concentration of pro-inflammatory cytokines IL-1β and TNFα and expression of tissue factor by peripheral blood monocytes in healthy individuals and patients with erysipelas in Transbaikalian region.
Methods: The study enrolled 104 patients with erysipelas and 94 healthy residents. Tissue factor gene polymorphism was detected by PCR method. Expression of tissue factor by monocytes was studied by the method of R.A. Santucci et al. (2000) in our modification.
Results: At was established that of homo- and heterozygous SNP of TF gene were spreading to Hardy-Weinberg equilibrium (p>0.05). It was founded that alleles and genotypes of SNP TF A603G, C1322T, C1812T, G1442C did not differ in healthy individuals and patients with erysipelas. Alleles and genotypes of SNP of TF gene didn´t effect on tissue factor expression. In the same time there was a significant difference between stimulated bacterial LPS and unstimulated blood samples in healthy individuals. But the difference was decreased significantly in patients with erysipelas (p < 0.001), which indicates a high expression of tissue factor. At was established that a decrease in blood coagulation time occurred with an increase in the concentration of IL-1β and TNFα in patients with erysipelas, which also indicates a high expression of tissue factor.
Conclusions: Thus, the concentration of pro-inflammatory cytokines IL-1β and TNFα influence on TF activity in healthy individuals and patients with erysipelas.
To cite this abstract in AMA style:
Emelyanov A, Emelyanova A, Markovsky A, Avetisyan M, Vitkovsky Y. Pro‑Inflammatory Cytokines, Tissue Factor Gene Polymorphism (A603G, C1322T, C1812T, G1442C) and Tissue Factor Expression in Patient with Erysipelas [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/pro%e2%80%91inflammatory-cytokines-tissue-factor-gene-polymorphism-a603g-c1322t-c1812t-g1442c-and-tissue-factor-expression-in-patient-with-erysipelas/. Accessed September 24, 2023.« Back to ISTH 2020 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/pro%e2%80%91inflammatory-cytokines-tissue-factor-gene-polymorphism-a603g-c1322t-c1812t-g1442c-and-tissue-factor-expression-in-patient-with-erysipelas/