ISTH Congress Abstracts

Official abstracts site for the ISTH Congress

MENU 
  • Home
  • Congress Archive
    • ISTH 2022 Congress
    • ISTH 2021 Congress
    • ISTH 2020 Congress
  • Resources
  • Search

Production of platelets in vitro in functionalised 3-dimensional scaffolds mimicking the bone marrow niche

H. Foster1, M. Colzani1, G. Bouet Chalon1, D. Howard1, C. Di Buduo2, N. Muller-Sienerth3, A. Waller1, Y. Sun4, J. Shepherd5, A. Evans1, P. Soprano6, M. Parsons7, Y. Ying Sims8, E. Turro9, P. Maguire10, S. Best5, R. Cameron5, A. Balduini11, G. Wright3, C. Ghevaert12

1Wellcome-MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge, England, United Kingdom, 2Department of Molecular Medicine, University of Pavia, Pavia, Italy, Pavia, Lombardia, Italy, 3Cell Surface Signalling Laboratory, Wellcome Trust Sanger Institute, Cambridge, England, United Kingdom, 4University of Birmingham, Birmingham, England, United Kingdom, 5Cambridge Centre for Medical Materials, University of Cambridge, Cambridge, England, United Kingdom, 6Department of Molecular Medicine, University of Pavia, Pavia, Italy, Pavía, Lombardia, Italy, 7University College Dublin, Dublin, Dublin, Ireland, 8Wellcome Trust Sanger Institute, Cambridge, England, United Kingdom, 9Icahn School of Medicine at Mount Sinai, New York, New York, United States, 10University College Dublin, Ireland, Dublin, Dublin, Ireland, 11University of Pavia, Pavia, Italy, Pavía, Lombardia, Italy, 12University of Cambridge, Cambridge, England, United Kingdom

Abstract Number: PB0354

Meeting: ISTH 2022 Congress

Theme: Platelets and Megakaryocytes » Megakaryocytes and Thrombopoiesis

Background: The safety, quality and supply of donor-derived platelet units intended for transfusion have improved over the past decades but significant problems still remain. In vitro-derived platelets offer a possible alternative but up-scaling production is hindered by our limited understanding of thrombopoiesis (the release of platelets by their mother cell, the megakaryocyte)

Aims: Here, we have developed an integrated strategy aiming to mimic ex vivo the bone marrow physiological niche that promotes thrombopoiesis by mature megakaryocytes in order to identify key elements that promote platelet production.

Methods: Proteomic and genomic analysis was employed to analyse differential expression levels of transmembrane proteins in cells that were shown to promote platelet production through direct contact with megakaryocytes. A panel of 259 recombinant biotinylated-transmembrane proteins were immobilised on streptavidin coated surfaces to screen in order to identify proteins that promoted platelet production.

Results: ACVR1B, CRTAM, MUCEN and BTN1A1 were identified to improve platelet production from either cord blood- (ACVR1B) or pluripotent stem cells-derived (CRTAM, MUCEN and BTN1A1) megakaryocytes. Using two different methodologies, we functionalise either collagen- or silk-based 3-dimensional scaffolds and confirm increased functional platelet production by up to two-fold.

Conclusion(s): This unbiased approach has allowed us to identify novel proteins whose role in platelet formation was previously unknown and highlights the potential gain of recreating the MK niche to allow in vitro platelets to become a viable alternative for transfusion.

To cite this abstract in AMA style:

Foster H, Colzani M, Bouet Chalon G, Howard D, Di Buduo C, Muller-Sienerth N, Waller A, Sun Y, Shepherd J, Evans A, Soprano P, Parsons M, Ying Sims Y, Turro E, Maguire P, Best S, Cameron R, Balduini A, Wright G, Ghevaert C. Production of platelets in vitro in functionalised 3-dimensional scaffolds mimicking the bone marrow niche [abstract]. https://abstracts.isth.org/abstract/production-of-platelets-in-vitro-in-functionalised-3-dimensional-scaffolds-mimicking-the-bone-marrow-niche/. Accessed November 29, 2023.

« Back to ISTH 2022 Congress

ISTH Congress Abstracts - https://abstracts.isth.org/abstract/production-of-platelets-in-vitro-in-functionalised-3-dimensional-scaffolds-mimicking-the-bone-marrow-niche/

Simple Search

Supported By:

Takeda logo

ISTH 2022 Congress site

Visit the official web site for the ISTH 2022 Virtual Congress »

  • Help & Support
  • About Us
  • Cookies & Privacy
  • Wiley Job Network
  • Terms & Conditions
  • Advertisers & Agents
Copyright © 2023 John Wiley & Sons, Inc. All Rights Reserved.
Wiley