Abstract Number: PB0466
Meeting: ISTH 2020 Congress
Background: Early diagnosis of chronic kidney disease (CKD) is necessary in preventing complications that develop into kidney failure and vascular complications. Currently, the detection of early stages of CKD is lacking predictive values, delaying the application of specific diagnostics and therapeutic interventions. End-stage renal disease (ESRD) patients are at risk for cardiovascular complications and vascular thrombosis, exhibiting numerous coagulation and fibrinolytic abnormalities.
Aims: Profiling inflammatory biomarkers and coagulation factors in ESRD patients may allow early onset detection and severity of kidney damage, furthering the understanding of the pathogenesis of this disease.
Methods: Citrated blood samples were collected from 95 confirmed ESRD patients undergoing maintenance hemodialysis under an IRB approved protocol. Biomarkers of inflammation and coagulation, including PAI-1, TAFI, CRP, MMP-9, MP, D-Dimer, Factor VII, IX, X, and XIII were measured using commercially available ELISA methods. Normal controls were comprised of commercially available male and female citrated plasma (George King Biomedical). All results were compiled as mean ± SEM, and percent change in comparison to normal human plasma.
Results: Biomarkers including CRP (7ug/ml ± 0.6, 183%), MMP-9 (88ng/ml ±8, 58%) D-Dimer (1059ng/ml±138), Factor IX (124%±3, 23%), and Factor XIII (104%±1, 27%) showed significant increase when compared to normal plasma. Elevated CRP indicates severe inflammatory response and elevated D-Dimer indicates endogenous fibrin formation. MP and PAI-1 suggest endothelial dysfunction.
Conclusions: These results indicate that the upregulation of inflammatory responses and hemostatic defects contribute to the multifactorial pathophysiology of ESRD. Profiling of these biomarkers may be helpful in the prognostic management of these patients.
To cite this abstract in AMA style:Bontekoe E, Grewal A, Bansal V, Ben Hadj Tahar M, Hoppensteadt D, Fareed J. Profiling of Inflammatory Biomarkers and Coagulation Factors in End-stage Renal Disease [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/profiling-of-inflammatory-biomarkers-and-coagulation-factors-in-end-stage-renal-disease/. Accessed October 1, 2023.
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