Abstract Number: PB0049
Meeting: ISTH 2021 Congress
Background: Rapid detection of DOAC levels and effective reversal in patients would aid clinical decisions. Point-of-Care (POC) storage-stable microfluidic chips offer rapid whole blood testing.
Aims: Evaluate an injection-molded 8-channel microfluidic chip for POC detection of DOACs in DVT patients utilizing the response of whole blood to ex vivo dosing of DOAC reversal agents.
Methods: Using a 1”x3” device with 8-parallel flow paths crossing a bio-printed surface of collagen/tissue factor (200 s-1 wall shear rate), we measured platelets and fibrin accumulation with time using CTI-treated blood from DVT patients (N=7). Platelet and fibrin clotting was imaged for blood that was unmodified or treated ex vivo with the reversal agent Andexxa at two different doses or high-dose FXa inhibitor.
Results: For whole blood clotting at a wall shear rate of 200 s-1, a statistically significant difference was observed between channels with 300 nM of reversal agent Andexanet Alfa present when compared with unaltered blood for 7 DVT patients on apixaban or rivaroxaban. Fluorescence intensity in the fibrin channel was increased in a dose dependent manner by the presence of the reversal agent. By evaluating the ratio of a patient’s untreated and anticoagulant-reversed blood and comparing with a known dose response curve for DOACs in this assay, patient drug concentrations can be predicted quantitatively
Conclusions: We demonstrate the ability to detect the presence of DOACs in whole blood samples drawn from DVT patients on oral Xa inhibitors.
To cite this abstract in AMA style:Rossi JM, Panckeri KA, Cuker AC, Diamond SL. Rapid Detection of DOACs in DVT Patients Using a Single-use Microfluidic Chip under Whole Blood Flow [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 1). https://abstracts.isth.org/abstract/rapid-detection-of-doacs-in-dvt-patients-using-a-single-use-microfluidic-chip-under-whole-blood-flow/. Accessed September 25, 2021.
« Back to ISTH 2021 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/rapid-detection-of-doacs-in-dvt-patients-using-a-single-use-microfluidic-chip-under-whole-blood-flow/