Abstract Number: PB0307
Meeting: ISTH 2020 Congress
Theme: Coagulation and Natural Anticoagulants » Critical Care and Perioperative
Background: Timely plasma transfusion is a critical part of hemostatic resuscitation during hemorrhage. Rapid thaw plasma (RTP) is a unit of plasma collected by apheresis and transferred in its pre-frozen liquid state into a 2000mL transfer bag that is also approved for plasma storage (TerumoBCT, Lakewood, CO) versus standard 400mL storage bags (Figure 1). Placing plasma into this large bag and freezing in a horizontal position creates a “paper thin” unit with significantly increased surface area, allowing RTP units to thaw more quickly versus standard plasma units.
Aims: Compare thaw times and major coagulation tests between RTP and standard plasma [plasma frozen within 24 hours of collection (FP24)] on both the day of thaw (Day 1) and Day 5 post-thaw.
Methods: Group A apheresis ACDA plasma were collected as 5 units of RTP and 5 units of randomly selected FP24, frozen, and stored at -30°C until ready for thawing. On Day 1, all units were thawed using the Barkey plasmatherm thawer (Barkey GmbH and Co., Leopoldshöhe, Germany). All 10 units were subsequently refrigerated at 1-6°C. On both Days 1 and 5, aliquots were drawn from each unit into a polypropylene transfer tube, re-frozen, and stored at -30°C. After subsequent thawing at 37°C, ACL Top 750 instruments with associated reagents (Instrumentation Laboratory, Bedford, MA) were utilized to perform coagulation testing on samples from Days 1 and 5, including PT/INR, PTT, Fibrinogen, and Factors V and VIII.
Results: RTP thawed in < 5 minutes for all units versus 18-28 minutes for FP24 (p< 0.0005). Coagulation test results demonstrated no significant differences between the products on Day 1 or 5 (Figure 2).
Conclusions: RTP thaws in < 5 minutes and maintains a similar coagulation profile to FP24 over 5 days of refrigerated storage. These findings support RTP as a superior product for rapid delivery of plasma.
[Figure 1. (A) Rapid Thaw Plasma (RTP) (left) and Standard Plasma (FP24) (right) Units (B) Cross Sectional View (RTP)]
[Figure 2. Coagulation Assays for Rapid Thaw Plasma and standard FP24 plasma (n=5 for each)]
To cite this abstract in AMA style:
Madden KM, Raval JS, Rosenbaum L, Richards C, Jones C, Lowery P, Rollins-Raval MA. Rapid Thaw Plasma Units Thaw Significantly Faster than Standard Plasma Units while Retaining Similar Coagulation Profile over 5 Days of Cold Storage [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/rapid-thaw-plasma-units-thaw-significantly-faster-than-standard-plasma-units-while-retaining-similar-coagulation-profile-over-5-days-of-cold-storage/. Accessed October 1, 2023.« Back to ISTH 2020 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/rapid-thaw-plasma-units-thaw-significantly-faster-than-standard-plasma-units-while-retaining-similar-coagulation-profile-over-5-days-of-cold-storage/