Abstract Number: PB0601
Meeting: ISTH 2020 Congress
Background: The ST Genesia is a benchtop, fully automated thrombin generation (TG) assay, related to the Calibrated Automated Thrombinography (CAT) assay. The ST Genesia is more standardized and ensures a stable and uniformly heat distribution throughout the measurement and the measuring cuvette.
Aims: To obtain reference values for the ST Genesia with all the reagents available. A comparison between men and women with/without oral contraceptives (OC) was evaluated.
Methods: Plasma samples from 117 healthy donors were measured on the ST Genesia with all three available reagents: STG-BleedScreen, STG-DrugScreen and STG-ThromboScreen. All reagents included at least two quality controls and a reference plasma. This reference plasma is used to normalize data and enables comparison between laboratories, between lots and eases multicenter studies. STG-ThromboScreen has a second trigger containing thrombomodulin (TM), to include the effect of the protein C pathway. One-way ANOVA was used to compare means of the three donor groups. Reference ranges limits were established with 2.5th-97th percentiles on both normalized and absolute TG parameters.
Results: Out of 117 donors, 49% were men, 38% women without OC and 13% women with OC, with an overall mean age (±SD) of 35 years (±12). Men and women without OC had, respectively, a mean peak height of 167nM and 164nM with STG-BleedScreeen, 335nM and 351nM with STG-DrugScreen and 192nM and 198nM with STG-ThromboScreen reagent. Women taking OC had a mean peak height of 263nM, 473nM and 312nM, respectively (p< 0.05). Interindividual variation for ETP was 23% for all reagents (similar to CAT). TM addition decreased ETP by 54% in men, 47% in women without OC and only 25% in women with OC (p< 0.05).
Conclusions: TG in men and women without OC was similar, however, significantly higher TG values were observed in women taking OC. TM addition decreased ETP significantly less in women taking OC.
To cite this abstract in AMA style:Ninivaggi M, de Laat-Kremers R, Carlo A, de Laat B. Reference Values of 117 Healthy Donors on the ST Genesia with the STG-BleedScreen, STG-DrugScreen and STG-ThromboScreen Reagents [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/reference-values-of-117-healthy-donors-on-the-st-genesia-with-the-stg-bleedscreen-stg-drugscreen-and-stg-thromboscreen-reagents/. Accessed October 2, 2023.
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