Abstract Number: PB0145
Meeting: ISTH 2020 Congress
Background: Plasma D-Dimer (D2D) is a degradation product of cross-linked fibrin and represents the activation of the fibrinolytic and coagulation system. Clinically, D2D tests have a high negative prediction value for thrombotic events and can be used to rule-out venous thromboembolism (VTE)1. The D2D cut-off value for VTE in humans is 500ng/mL; however, the baseline and cut-off values for rats are unknown.
Aims: To systematically evaluate the reported results and methodology of D2D tests on rat models.
Methods: A systematic literature search was conducted using MEDLINE, EMBASE and Web of Science to include relevant full-length publications, published abstracts and conference proceedings from Jan-01-2000 to Dec-20-2019 that reported rat D2D values. The search strategy used categorical search terms: “rat” AND “D-dimer” OR “fibrin degradation product”. Eligible articles were independently reviewed for strain, age, sex, baseline D2D and measurement methodology.
Results: Of the 520 identified records 111 studies were included for final analysis. The kappa interrater agreement for study inclusion before resolution was 0.94. Twelve strains with a body mass ranging from 70-410 grams and of both sexes were included in the analysis. Figure 2 illustrates baseline D2D levels stratified by strain and methodology. There was a significant difference in reported baseline D2D between rat strains (P= 0.005). Despite large variances in reported baseline D2D between measurement methodologies, the differences were not statistically significant.
Conclusions: Large discrepancies in reported rat plasma D2D values suggest that factors such as species and detection methods can lead to the variation of results and should be considered when designing a rat model that measures D2D. We recommend using related negative control models as a baseline D2D reference range for each study aiming to measure D2D level in rats. Further research is required to establish a standardized reference range for baseline D2D levels to help scientists better interpret rat D2D test results.
To cite this abstract in AMA style:Chung J, Afraz S, Matino D, Chan A. Reported Values and Methodologies for Detecting Plasma D-dimer in Rat Models: A Systematic Review and Meta-analysis [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/reported-values-and-methodologies-for-detecting-plasma-d-dimer-in-rat-models-a-systematic-review-and-meta-analysis/. Accessed February 27, 2024.
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