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Reticulated Platelets in Healthy Volunteers. Relationships with Platelet Size Indexes and Platelet Activity

A. Mazurov1, V. Bodrova1, O. Shustova1, S. Khaspekova1

1National Medical Research Center of Cardiology, Moscow, Russian Federation

Abstract Number: PB1000

Meeting: ISTH 2021 Congress

Theme: Platelets and Megakaryocytes » Platelet Function and Interactions

Background: Reticulated platelets (RP) are young, functionally active platelet forms which are detected by RNA staining. Their content in circulation reflects the intensity of thrombocytopoesis in the bone marrow.

Aims: In this study we assessed the relationships of RP content with platelet size and activity in healthy volunteers.

Methods: RP were evaluated by thiazole orange staining using flow cytometry, platelet size indexes included mean platelet volume (MPV), platelet large cell ratio (P-LCR) measured in a Coulter type analyzer and forward scattering (FSC) measured in a flow cytometer. Platelet activity was evaluated by expression of activated glycoprotein (GP) IIb-IIIa (PAC-1 antibody binding) and CD62P using flow cytometry. Platelets were activated by thrombin receptor activating peptide (TRAP) (10 and 1 µM) and ADP (20 and 2.5 µM).

Results: The percentage of RP in healthy volunteers varied from 2.9% to 23.8% (mean ± SD – 11.7 ± 4.7%, n = 99) and correlated with all platelet size indexes, MPV, P-LCR and FCS (r from 0.452 to 0.529, p < 0.001, n = 87-99). RP were distributed at a ratio 9:1 between 50% subpopulations of large and small platelets according to FSC index. Expression of activated GP IIb-IIIa correlated with RP percentage and platelet size indexes when platelets were activated by both doses of TRAP and ADP (r from 0.309 to 0.560, p from 0.014 to < 0.001, n = 50- 62). CD62P expression correlated with RP percentage and platelet size indexes when platelets were activated by 10 µM TRAP inducing maximal CD62P expression, (r from 0.332 to 0.556, p from 0.008 to < 0.001, n = 65), but not by weaker agonists, 1 µM TRAP and both ADP doses (r < 0.3, n = 54-66).

Conclusions: High RP content in healthy volunteers is associated with the increase of platelet size and activity in the whole platelet population

To cite this abstract in AMA style:

Mazurov A, Bodrova V, Shustova O, Khaspekova S. Reticulated Platelets in Healthy Volunteers. Relationships with Platelet Size Indexes and Platelet Activity [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 2). https://abstracts.isth.org/abstract/reticulated-platelets-in-healthy-volunteers-relationships-with-platelet-size-indexes-and-platelet-activity/. Accessed May 19, 2022.

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