Abstract Number: PB1323
Meeting: ISTH 2020 Congress
Background: Numeric and functional defects of Ts cells and immune regulation abnormalities of mesenchymal stem cells (MSCs) are involved in immune thrombocytopenia (ITP) pathogenesis. Recent studies showed that MSCs can promote Ts cell differentiation.
Aims: comparing the Ts cell induction abilities of bone marrow-derived MSCs (BM-MSCs) of patients with ITP and normal controls (NCs) to examine the causes of Ts cell defects, MSCs immune regulation abnormalities and the reason behind this difference.
Methods: BM-MSCs were isolated from 16 ITP patients and 10 normal controls, after co-cultured of CD8+ T cells with BM-MSCs for 3 days, CD8+CD28- percentages, FasL , IL-10, CD25 , CD69 expression on Ts or effector T cells, along with cell apoptosis and proliferation were analyzed by FACS; and inhibition function of Ts cell was detected by multi-lymphocyte reactions. Moreover, UC-MSCs were co-cultured with Ts cells (1:20) from ITP patients to determine whether they can repair Ts defects in ITP patients.
Results: BM-MSCs elevated Ts cell percentage and function, but the efficiency was lower in patients with ITP than in NCs. Neutralization or blockade experiments showed that blockade of interleukin (IL)-6 partially reversed Ts cell induction by BM-MSCs. Addition of exogenous IL-6 down-regulated Ts cell apoptosis. Moreover, BM-MSCs enhanced IL-10 secretion from Ts cells and increased Ts cell ability to inhibit effector T cell activation. IL-6 secretion, regulatory abilities of IL-10 expression in Ts cells, and the enhanced efficiency of Ts cells to inhibit effector T cell activation by BM-MSCs were all decreased in patients with ITP. And umbilical cord-derived MSCs (UC-MSCs) efficiently improved ITP Ts cell numbers and dysfunction.
Conclusions: Defects in BM-MSCs involved in Ts cell induction play a role in ITP pathogenesis, and exogenous UC-MSCs may be useful for ITP therapy.
To cite this abstract in AMA style:Li H, Yang R. Role of Bone Marrow-Derived Mesenchymal Stem Cell Defects in CD8+ CD28- Suppressor T-Lymphocyte Induction in Patients with Immune Thrombocytopenia and Associated Mechanisms [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/role-of-bone-marrow-derived-mesenchymal-stem-cell-defects-in-cd8-cd28-suppressor-t-lymphocyte-induction-in-patients-with-immune-thrombocytopenia-and-associated-mechanisms/. Accessed March 4, 2024.
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