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Role of Low-dose Aspirin on the Release of Circulating Microvesicles in Patients at Cardiovascular Risk with and without Type 2 Diabetes

P. Simeone1, R. Liani1, R. Tripaldi1, S. Ciotti1, P. Lanuti1, M. Marchisio1, S. Miscia1, F. Santilli1

1Department of Medicine and Aging, Center for Advanced Studies and Technology (CAST), University "G. d'Annunzio" of Chieti-Pescara, Chieti, Italy

Abstract Number: LPB0097

Meeting: ISTH 2021 Congress

Theme: Arterial Thromboembolism » Atherosclerosis

Background: Microvesicles(MVs) are small vesicles deriving from all cell type. MVs seems to be one of the procoagulant determinants in type 2 diabetes(T2DM).

Aims: To assess the effects of chronic low dose aspirin(ASA) on levels of total MVs and specific subtypes, such as platelet(PMV), endothelial(EMV), leucocytes(LMV) in DM and non-DM patients in the 24-hour interval between 2 witnessed ASA administrations.

Methods: We enrolled 59 patients with and 41 without T2DM, at cardiovascular risk, on chronic low-dose ASA treatment. The kinetics of platelet cyclooxygenase-1 recovery(COX-1) was characterized by measuring serum TXB2 after 10 and 24 hours after a witnessed ASA administration. Nine healthy subjects were enrolled to verify the number of MVs on circadian measurements. Each subject signed written informed consent. Protocol was approved.

Results: Total MVs levels and the number of CD45+ LMV were reduced at T10 as compared to T24 within both DM(p <0.001 and p=0.009, respectively) and noDM patients(p <0.001 and p=0.034) and in the two groups(p <0.001 both).The CD41a+PMVs and CD31+EMV did not show differences between T10 and T24 in DM and in non-DM patients, whereas reduced levels at T10 vs. T24 were observed for Annex-positive CD41a+ PMVs . In healthy subjects, nodifference in the levels of total MV, PMV and EMV was observed between T10 and T24, over 24 h between two ASA administrations.
Levels of Total Microvescicles, and Platelet derived Microvescicles Annex positive in patients with and without diabetes after 10 and 24 hours after a witnessed aspirin administration

Conclusions: In ASA-treated subjects, daily administration may inhibit the release of total MV and annexin-positive, phosphatydilserine -exposing, PMVduring the 24-hour dosing interval. After excluding a circadian variation in MV levels, mirrored by the stability of the phenotype in healthy subjects, the  inhibition after 10 hours since a witnessed ASA administration suggests that COX-1 dependent mechanisms may be involved in this inhibition. With the limits of a cross-sectional study, our findings suggest a previously unappreciated effect of ASA. 
 

To cite this abstract in AMA style:

Simeone P, Liani R, Tripaldi R, Ciotti S, Lanuti P, Marchisio M, Miscia S, Santilli F. Role of Low-dose Aspirin on the Release of Circulating Microvesicles in Patients at Cardiovascular Risk with and without Type 2 Diabetes [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 2). https://abstracts.isth.org/abstract/role-of-low-dose-aspirin-on-the-release-of-circulating-microvesicles-in-patients-at-cardiovascular-risk-with-and-without-type-2-diabetes/. Accessed May 20, 2022.

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