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Role of Platelet’s Heme Oxygenase-1 in Obese Patients with and without Type 2 Diabetes Mellitus

1Department of Medicine and Aging, and Center for Advanced Studies and Technology (CAST), ‘G. d'Annunzio‘ University of Chieti-Pescara, Chieti, Italy, 2Department of Drug Sciences, University of Catania, Catania, Italy

Abstract Number: PB0981

Meeting: ISTH 2021 Congress

Theme: Platelets and Megakaryocytes » Platelet Function and Interactions

Background: Oxidative stress plays a pivotal role in the development of microvascular and cardiovascular complications. Obesity is accompanied by a state of chronic, low-grade, systemic inflammation that increases risk for cardiovascular disease (CVD) exacerbating the vascular inflammatory response. High levels of reactive oxygen species (ROS) are linked to obesity and to type 2 diabetes mellitus (T2DM). Enhanced heme turnover catalyzed by heme oxygenase (HO)-1, an inducible enzyme, induce anti-inflammatory, antioxidant, and anti-apoptotic functions.

Aims: To determine in a pathological context with a high oxidative stress and prothrombotic risk, as obesity and T2DM, the presence and role of HO-1 in platelet and its release in extracellular compartment.

Methods: Fifty-one obese (OB) (31 male, median age 67 years) and sixty-six non-OB patients (40 male, median age 69 years), with or without T2DM, were enrolled. Blood samples were collected at 8 am after an overnight fasting. PLT’s HO-1 was assayed with ELISA kit (EnzoLife). Microvesicles (MVs) were analyzed on fresh sample with CFM. Intima–media thickness (IMT) was obtained with Doppler system (GE Healthcare). Each subject signed written informed consent, and protocol was approved (GR-2011-02350450).

Results: PLT HO-1 levels were significantly higher in non-OB vs. OB patients (all, p=0.0089; noT2DM, p=0.015 Fig. 1). PLT’s MVs were significantly lower in non-OB patients (all, p=0.017; noT2DM, p=0.023, Fig. 1) and correlated inversely with HO-1 in non-OB patients (all, rho =-0.486, p=0.025; non-OB NOT2DM rho=-0.857, p=0.001 Fig.1).
Indeed, HO-1 correlated inversely with IMT in non-OB patients (sx: all, rho=-0.428, p=0.003, noT2DM, rho=-0.491, p=0.009; dx, all, rho=-0-342 p=0.021, noT2DM, rho=-0.418, p=0.030, Fig. 2).PLT’s HO-1 and microvesicle
PLT’s HO-1 and medium_IMT

Conclusions: PLT levels of HO-1 appear to be closely related to the number of MVs of platelet origin, suggesting a role of MVs in its extra-PLT transport. Higher PLT levels of HO-1 may reflect a lower atherothrombotic risk, to further underline its protective role in cardiovascular diseases.

To cite this abstract in AMA style:

Liani R, Simeone PG, Tripaldi R, Ciotti S, Lanuti P, Bologna G, Boccatonda A, D'Ardes D, Luca V, Santilli F. Role of Platelet’s Heme Oxygenase-1 in Obese Patients with and without Type 2 Diabetes Mellitus [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 2). https://abstracts.isth.org/abstract/role-of-platelets-heme-oxygenase-1-in-obese-patients-with-and-without-type-2-diabetes-mellitus/. Accessed May 20, 2022.

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